PHOSPHO1 Protein, Human (His)

PHOSPHO1 Protein, a phosphatase, is crucial in generating inorganic phosphate for bone mineralization, displaying high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). It collaborates with PHOSPHO1 in skeletal mineralization, initiating hydroxyapatite crystallization in matrix vesicles (MVs), while ALPL/TNAP catalyzes hydroxyapatite crystallization in the extracellular matrix. PHOSPHO1 Protein, Human (His) is the recombinant human-derived PHOSPHO1 protein, expressed by E. coli , with N-His labeled tag. The total length of PHOSPHO1 Protein, Human (His) is 267 a.a., with molecular weight of ~32 kDa.

PHOSPHO1 Protein is a phosphatase with high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho), playing a crucial role in the generation of inorganic phosphate for bone mineralization. It collaborates with PHOSPHO1 in skeletal mineralization, where PHOSPHO1 initiates hydroxyapatite crystallization in matrix vesicles (MVs), and ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

Q8TCT1 (M1-C267)

162466  [NCBI]

MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDENSDDSIVRAAPGQRLPESLRATYREGFYNEYMQRVFKYLGEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEVILISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGLLALRPFHTHSCARCPANMCKHKVLSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEPSSFRASVVPWETAADVRLHLQQVLKSC

Approximately 32 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, 300mM L-Arginine, pH 7.0 or 20 mM PB, 200 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.