rLuc Protein, Human (His, Strep)
rLuc Protein, Human (His, Strep) is the recombinant human-derived rLuc, expressed by E. coli , with Strep, His labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
rLuc Protein, Human (His, Strep) is the recombinant human-derived rLuc, expressed by E. coli , with Strep, His labeled tag. ,
Renilla Luciferase is an enzyme that, upon binding its substrate, catalyzes an oxygenation reaction, producing a highly unstable hydroperoxide that cyclizes into a dioxetanone structure. The dioxetanone then collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases approximately 50 kcal/mol of energy, which is required to generate the excited state of the coelenteramide product (the singlet form of the monoanion). In vivo, the product undergoes nonradiative energy transfer to an accessory protein, green fluorescent protein (GFP), resulting in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Technical Parameters
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Species Human
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Source E. coli
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Tag Strep;His
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Accession
P27652 (M1-Q311)
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Gene ID/
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Protein Length
Full Length
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Synonyms
Coelenterazine h 2-monooxygenase; Renilla-luciferin 2-monooxygenase; Renilla-type luciferase; Renilla reniformis; Sea pansy; Decarboxylase; Lyase; Monooxygenase; Oxidoreductase; Photoprotein; 1.13.12.5
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AA Sequence
MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ
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Predicted Molecular Mass
39.2 kDa
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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