RNF43 Protein, Canine (HEK293, His)
RNF43 Protein, Canine (HEK293, His) is the recombinant canine-derived RNF43 protein, expressed by HEK293, with C-His tag.
- Species: Canine
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
RNF43 Protein, Canine (HEK293, His) is the recombinant canine-derived RNF43 protein, expressed by HEK293, with C-His tag.
Background
The RNF43 gene encodes a RING-type E3 ubiquitin ligase, predicted to encompass a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is implicated in the negative regulation of Wnt signaling, exerting its influence through increased ubiquitination of frizzled receptors and inducing alterations in their subcellular distribution, ultimately resulting in reduced surface levels of these receptors. Notably, mutations in this gene have been identified in various tumor cells, including those associated with colorectal and endometrial cancers. The gene exhibits broad expression, with notable levels observed in the duodenum (RPKM 7.1), colon (RPKM 6.1), and 24 other tissues. The multifaceted role of RNF43 highlights its significance in modulating critical cellular pathways and its potential implications in tumorigenesis.
Technical Parameters
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Species Canine
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Source HEK293
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Tag C-8*His
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Accession
XP_038533015 (G24-Y197)
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Gene ID54894
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Protein Length
Partial
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Synonyms
RNF43; DKFZp781H0392; Ring Finger Protein 43; FLJ20315; RNF124; RING-Type E3 Ubiquitin Transferase; URCC; RING Finger Protein 43; RING-Type E3 Ubiquitin Transferase RNF43; SSPCS; E3 Ubiquitin-Protein Ligase RNF43
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AA Sequence
GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPQRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNRKAHVRIELKESPTWPDY
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Predicted Molecular Mass
20.7 kDa
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Molecular Weight
Approximately 30-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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