RNF43 Protein, Canine (HEK293, His)

RNF43 Protein, Canine (HEK293, His) is the recombinant canine-derived RNF43 protein, expressed by HEK293, with C-His tag.

The RNF43 gene encodes a RING-type E3 ubiquitin ligase, predicted to encompass a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is implicated in the negative regulation of Wnt signaling, exerting its influence through increased ubiquitination of frizzled receptors and inducing alterations in their subcellular distribution, ultimately resulting in reduced surface levels of these receptors. Notably, mutations in this gene have been identified in various tumor cells, including those associated with colorectal and endometrial cancers. The gene exhibits broad expression, with notable levels observed in the duodenum (RPKM 7.1), colon (RPKM 6.1), and 24 other tissues. The multifaceted role of RNF43 highlights its significance in modulating critical cellular pathways and its potential implications in tumorigenesis.

Canine

HEK293

C-8*His

XP_038533015 (G24-Y197)

Partial

GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPQRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNRKAHVRIELKESPTWPDY

Approximately 30-40 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.