RP105/CD180 Protein, Human (HEK293, His)
RP105/CD180 is a key mediator in B cells that cooperates with MD-1 and TLR4 to initiate the innate immune response against bacterial lipopolysaccharide (LPS). This cooperation activates NF-kappa-B, underscoring its role in key signaling pathways. RP105/CD180 Protein, Human (HEK293, His) is the recombinant human-derived RP105/CD180 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
RP105/CD180 is a key mediator in B cells that cooperates with MD-1 and TLR4 to initiate the innate immune response against bacterial lipopolysaccharide (LPS). This cooperation activates NF-kappa-B, underscoring its role in key signaling pathways. RP105/CD180 Protein, Human (HEK293, His) is the recombinant human-derived RP105/CD180 protein, expressed by HEK293 , with C-His labeled tag.
Background
The RP105/CD180 protein emerges as a key player in mediating the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells, likely through cooperation with MD-1 and TLR4. This collaborative effort leads to NF-kappa-B activation, underscoring its role in initiating crucial signaling pathways. Additionally, RP105/CD180 is implicated in the life/death decision of B-cells, indicating its involvement in critical cellular processes. The structural organization of RP105/CD180 involves the formation of an M-shaped tetramer, constituted by two CD180-LY86 heterodimers. These distinctive features highlight the multifaceted nature of RP105/CD180 in orchestrating immune responses and cellular decisions in B-cells. Further exploration is essential to unravel the specific molecular mechanisms through which RP105/CD180 contributes to the intricate dynamics of the innate immune system and its collaboration with MD-1 and TLR4 in response to bacterial LPS.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q99467/NP_005573.2 (W24-G626)
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Molecular Construction
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N-term
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RP105 (W24-G626)
Accession # Q99467/NP_005573.2 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CD180; Ly78; Prev. LY64; Lymphocyte Antigen 64; RP105; Lymphocyte Antigen 64 (Mouse) Homolog, Radioprotective, 105kD; CD180 Antigen; Lymphocyte Antigen-64, Radioprotective, 105kDa; CD180 Molecule; Radioprotective 105 KDa Protein
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AA Sequence
WDQMCIEKEANKTYNCENLGLSEIPDTLPNTTEFLEFSFNFLPTIHNRTFSRLMNLTFLDLTRCQINWIHEDTFQSHHQLSTLVLTGNPLIFMAETSLNGPKSLKHLFLIQTGISNLEFIPVHNLENLESLYLGSNHISSIKFPKDFPARNLKVLDFQNNAIHYISREDMRSLEQAINLSLNFNGNNVKGIELGAFDSTIFQSLNFGGTPNLSVIFNGLQNSTTQSLWLGTFEDIDDEDISSAMLKGLCEMSVESLNLQEHRFSDISSTTFQCFTQLQELDLTATHLKGLPSGMKGLNLLKKLVLSVNHFDQLCQISAANFPSLTHLYIRGNVKKLHLGVGCLEKLGNLQTLDLSHNDIEASDCCSLQLKNLSHLQTLNLSHNEPLGLQSQAFKECPQLELLDLAFTRLHINAPQSPFQNLHFLQVLNLTYCFLDTSNQHLLAGLPVLRHLNLKGNHFQDGTITKTNLLQTVGSLEVLILSSCGLLSIDQQAFHSLGKMSHVDLSHNSLTCDSIDSLSHLKGIYLNLAANSINIISPRLLPILSQQSTINLSHNPLDCTCSNIHFLTWYKENLHKLEGSEETTCANPPSLRGVKLSDVKLSCG
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Predicted Molecular Mass
69 kDa
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Molecular Weight
Approximately 90-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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