SARS-CoV-2 Nucleocapsid Protein (G335A, sf9, His)
The SARS-CoV-2 nucleocapsid protein plays a crucial role in assembling the virion, forming the helical ribonucleocapsid (RNP), and interacting with the viral genome and membrane protein M. In addition to its structural function, it also enhances subgenomic viral RNA transcription and overall replication. SARS-CoV-2 Nucleocapsid Protein (G335A,sf9,His) is the recombinant SARS-CoV-2 Nucleocapsid protein, expressed by Sf9 insect cells, with C-His labeled tag.
- Species: Virus
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
The SARS-CoV-2 nucleocapsid protein plays a crucial role in assembling the virion, forming the helical ribonucleocapsid (RNP), and interacting with the viral genome and membrane protein M. In addition to its structural function, it also enhances subgenomic viral RNA transcription and overall replication. SARS-CoV-2 Nucleocapsid Protein (G335A,sf9,His) is the recombinant SARS-CoV-2 Nucleocapsid protein, expressed by Sf9 insect cells, with C-His labeled tag.
Background
The SARS-CoV-2 Nucleocapsid Protein assumes a pivotal role in virion assembly, orchestrating the packaging of the positive-strand viral genome RNA into a helical ribonucleocapsid (RNP) and establishing crucial interactions with the viral genome and membrane protein M. Beyond its structural functions, this protein significantly contributes to the efficiency of subgenomic viral RNA transcription and overall viral replication. Notably, it exhibits an additional role in modulating host chemokine function, a mechanism that may favor viral replication and transmission. The Nucleocapsid Protein achieves this modulation by being secreted into the extracellular space, where it competes with host chemokines for binding to host glycosaminoglycans (GAG), thus potentially disrupting host defense mechanisms and supporting the viral life cycle.
Technical Parameters
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Species Virus
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Source Sf9 insect cells
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Tag C-His
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Accession
YP_009724397.2 (M1-A419, G335A)
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Gene ID43740575
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Molecular Construction
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N-term
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Nucleocapsid (M1-A419, G335A)
Accession # YP_009724397.2 -
His
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C-term
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Protein Length
Full Length
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Synonyms
Nucleocapsid protein
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AA Sequence
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
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Predicted Molecular Mass
47.1 kDa
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Molecular Weight
Approximately 45-50 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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