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SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His)

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Based on 1 Customer Validation

Nucleoprotein/NP Protein is essential for viral replication and transcription. Nucleoprotein/NP Protein binds to viral RNA to form a ribonucleoprotein complex required for genome replication. Nucleoprotein/NP Protein promotes NLRP3 inflammasome activation to induce excessive inflammation. SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) is the recombinant Virus-derived SARS-CoV-2 Nucleocapsid protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Virus
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His)
  • Experimental Validation Results for SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His)
  • SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Nucleoprotein/NP Protein is essential for viral replication and transcription. Nucleoprotein/NP Protein binds to viral RNA to form a ribonucleoprotein complex required for genome replication. Nucleoprotein/NP Protein promotes NLRP3 inflammasome activation to induce excessive inflammation. SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) is the recombinant Virus-derived SARS-CoV-2 Nucleocapsid protein, expressed by E. coli , with N-His labeled tag.

Background

Nucleoprotein/NP Protein is essential for viral replication and transcription. Nucleoprotein/NP Protein binds to viral RNA to form a ribonucleoprotein complex required for genome replication. Nucleoprotein/NP Protein induces strong antibody (Ab) and T cell responses, regulating innate and adaptive immunity[1][3].
Nucleoprotein/NP Protein functions by secreting into the extracellular space, where it competes with host chemokines to bind to host glycosaminoglycans (GAGs)[2].
Nucleoprotein/NP Protein induces excessive inflammation by promoting the activation of the NLRP3 inflammasome, which plays a role in COVID-related lung injury[4].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Nucleocapsid at 1μg/mL (100μL/well) can bind Anti- SARS-CoV-2 Nucleocapsid antibody. The ED50 for this effect is 2.792 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Nucleocapsid at 1 μg/mL (100 μL/well) can bind Anti- SARS-CoV-2 Nucleocapsid antibody. The ED50 for this effect is 2.792 ng/mL.

Technical Parameters

  • Species Virus
  • Source E. coli
  • Tag N-His
  • Accession

    YP_009724397 (M1-A419, P13L, ΔE31-S33, R203K, G204R)

  • Gene ID
    43740575  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • Nucleocapsid (M1-A419, with multiple mutations)
      Accession # YP_009724397
    • C-term
  • Protein Length

    Full Length

  • Mutation

    P13L, ΔE31-S33, R203K, G204R

  • Mutation Definition

    P13L,ΔE31-S33, R203K, G204R: SARS-CoV-2 Omicron variant-associated mutations (Pango lineage B.1.1.529)

  • Synonyms

    N Protein; Nucleocapsid

  • AA Sequence

    MSDNGPQNQRNALRITFGGPSDSTGSNQNGGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA

  • Predicted Molecular Mass

    46.3 kDa

  • Molecular Weight

    Approximately 50 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM PB, 500 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Nucleocapsid at 1 μg/mL (100 μL/well) can bind Anti- SARS-CoV-2 Nucleocapsid antibody. The ED50 for this effect is 2.792 ng/mL.

  • SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) Product Image
    1/16
  • Experimental Validation Results for SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His)
  • Experimental Validation Results for SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His)
  • SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) Product Image