1. Recombinant Proteins
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 Nucleocapsid Proteins
  5. SARS-CoV-2 N Protein (NP)
  6. SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His)

SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His)

Cat. No.: HY-P74443
COA Handling Instructions

Nucleoprotein/NP Protein is essential for viral replication and transcription. Nucleoprotein/NP Protein binds to viral RNA to form a ribonucleoprotein complex required for genome replication. Nucleoprotein/NP Protein promotes NLRP3 inflammasome activation to induce excessive inflammation. SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) is the recombinant Virus-derived SARS-CoV-2 Nucleocapsid protein, expressed by E. coli , with N-His labeled tag. The total length of SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) is 419 a.a., with molecular weight of ~46.31 kDa.

For research use only. We do not sell to patients.

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Description

Nucleoprotein/NP Protein is essential for viral replication and transcription. Nucleoprotein/NP Protein binds to viral RNA to form a ribonucleoprotein complex required for genome replication. Nucleoprotein/NP Protein promotes NLRP3 inflammasome activation to induce excessive inflammation. SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) is the recombinant Virus-derived SARS-CoV-2 Nucleocapsid protein, expressed by E. coli , with N-His labeled tag. The total length of SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) is 419 a.a., with molecular weight of ~46.31 kDa.

Background

Nucleoprotein/NP Protein is essential for viral replication and transcription. Nucleoprotein/NP Protein binds to viral RNA to form a ribonucleoprotein complex required for genome replication. Nucleoprotein/NP Protein induces strong antibody (Ab) and T cell responses, regulating innate and adaptive immunity[1][3].
Nucleoprotein/NP Protein functions by secreting into the extracellular space, where it competes with host chemokines to bind to host glycosaminoglycans (GAGs)[2].
Nucleoprotein/NP Protein induces excessive inflammation by promoting the activation of the NLRP3 inflammasome, which plays a role in COVID-related lung injury[4].

Species

Virus

Source

E. coli

Tag

N-His

Accession

YP_009724397 (M1-A419)

Gene ID

43740575  [NCBI]

Molecular Construction
N-term
His
Nucleocapsid (M1-A419)
Accession # YP_009724397
C-term
Synonyms
N Protein; Nucleocapsid
Molecular Weight

Approximately 46.31 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM PB, 500mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
SARS-CoV-2 Nucleocapsid Protein (Omicron, B.1.1.529, His)
Cat. No.:
HY-P74443
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