SARS-CoV-2 RDRP Protein (sf9, His)

The function of viral RNA transcription and replication is performed by a specific RNA dependent RNA polymerase (RdRp) region present in ORF1ab, including non-structure proteins NSP12 with nucleotidyltransferase activity and NSP13 with a Zinc-binding domain involved in replication and transcription. The catalytic subunit (Nsp12) RdRp enzyme is the core component of multisubunit replication and transcription complex of NSPs. SARS-CoV-2 RDRP Protein (sf9, His) is the recombinant Virus-derived SARS-CoV-2 RDRP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of SARS-CoV-2 RDRP Protein (sf9, His) is 932 a.a., with molecular weight of ~91.3 kDa.

The function of viral RNA transcription and replication is performed by a specific RNA dependent RNA polymerase (RdRp) region present in ORF1ab, including non-structure proteins NSP12 with nucleotidyltransferase activity and NSP13 with a Zinc-binding domain involved in replication and transcription. A multisubunit replication and transcription complex of NSPs arbitrate the replication of SARS-CoV-2 The catalytic subunit (Nsp12) RdRp enzyme is the core component of this complex.Nsp12 has little activity by itself and requires additional factors (Nsp7 and Nsp8) for proper functioning^[1][2].

Data is not available. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Virus

Sf9 insect cells

C-His

YP_009725307 (S1-Q932)

43740578  [NCBI]

Approximately 91.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, 10% Glycerol, 22.5% Trehalose, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.