2. Recombinant Proteins
  3. Viral Proteins Biotinylated Proteins
  4. SARS-CoV-2 Proteins
  5. SARS-CoV-2 Spike Proteins
  6. SARS-CoV-2 S2 Protein
  7. SARS-CoV-2 S Protein NTD (Biotinylated, 303a.a, HEK293, His-Avi)

SARS-CoV-2 S Protein NTD (Biotinylated, 303a.a, HEK293, His-Avi)

Art. -Nr.: HY-P705082
Handling Instructions Technical Support

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  • Technical Parameters

  • Properties

  • Beschreibung

Species

Virus
Source

HEK293
Tag

C-Avi;C-His
Accession

QHD43416.1 (V16-F318)
Gene ID

43740568
Molecular Construction
N-term
Spike NTD (V16-F318)
Accession # QHD43416.1
His-Avi
C-term
Conjugation

Biotin
Synonyms
S1 protein NTD; Spike protein S1 NTD; BetaCoV S1-NTD
AA Sequence

VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNF

Predicted Molecular Mass
38.0 kDa.
Molekulargewicht

Approximately 53-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Glycosylation
Yes
Reinheit

≥ 90%, as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.
Formulation

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Versand

Room temperature in continental US; may vary elsewhere.
Dokumentation
Handling Instructions (2659 KB)

SARS-CoV-2 S Protein NTD (Biotinylated, 303a.a, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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