1. Recombinant Proteins
  2. Viral Proteins Biotinylated Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 Spike Proteins
  5. SARS-CoV-2 Trimeric S Protein
  6. SARS-CoV-2 S Trimer Protein (Biotinylated, HEK293, His-Avi)

SARS-CoV-2 S Trimer Protein (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78106
COA Handling Instructions

SARS-CoV-2 S1 Protein, in its Spike protein S1 form, initiates infection by attaching the virion to the cell membrane through host receptor interaction. Simultaneously, Spike protein S2' acts as a viral fusion peptide, revealing itself after S2 cleavage during virus endocytosis. SARS-CoV-2 S Trimer Protein (Biotinylated, HEK293, His-Avi) is the recombinant Virus-derived SARS-CoV-2 S Trimer protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of SARS-CoV-2 S Trimer Protein (Biotinylated, HEK293, His-Avi) is 1173 a.a., with molecular weight of 140-170 kDa.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

SARS-CoV-2 S1 Protein, in its Spike protein S1 form, initiates infection by attaching the virion to the cell membrane through host receptor interaction. Simultaneously, Spike protein S2' acts as a viral fusion peptide, revealing itself after S2 cleavage during virus endocytosis. SARS-CoV-2 S Trimer Protein (Biotinylated, HEK293, His-Avi) is the recombinant Virus-derived SARS-CoV-2 S Trimer protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of SARS-CoV-2 S Trimer Protein (Biotinylated, HEK293, His-Avi) is 1173 a.a., with molecular weight of 140-170 kDa.

Background

The SARS-CoV-2 S1 Protein plays a crucial role in the early stages of viral infection. Spike protein S1 facilitates the attachment of the virion to the cell membrane by interacting with host receptors, thereby initiating the infection process. This initial binding event is pivotal for the subsequent entry of the virus into the host cell. Concurrently, Spike protein S2', serving as a viral fusion peptide, comes into play after S2 cleavage during virus endocytosis. The unmasking of S2' is a key step in the viral fusion process, enabling the merging of the viral membrane with the endosomal membrane and facilitating the release of the viral genetic material into the host cell cytoplasm. The concerted action of these S1 and S2' functionalities underscores the significance of the SARS-CoV-2 S1 Protein in mediating viral entry and fusion, crucial steps in the viral life cycle.

Biological Activity

Immobilized Biotinylated SARS-COV-2 Spike S (Trimer) , His Tag at 2 μg/mL (100 μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human ACE2, hFc Tag with the EC50 of ≤ 91.1 ng/mL determined by ELISA.

Species

Virus

Source

HEK293

Tag

C-Avi;C-His

Accession

QHD43416 (V16-E1188)

Gene ID

43740568  [NCBI]

Synonyms
S protein; Spike glycoprotein; S glycoprotein; COVID-19
Molecular Weight

140-170 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

SARS-CoV-2 S Trimer Protein (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SARS-CoV-2 S Trimer Protein (Biotinylated, HEK293, His-Avi)
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HY-P78106
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