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Semaphorin-4A/SEMA4A Protein, Human (HEK293, His)

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Based on 1 Customer Validation

Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1 and is essential for intercellular signaling. It induces TIMD2 phosphorylation, inhibits angiogenesis, and collapses axonal growth cones. Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Semaphorin-4A/SEMA4A Protein, Human (HEK293, His)
  • Experimental Validation Results for Semaphorin-4A/SEMA4A Protein, Human (HEK293, His)
  • Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1 and is essential for intercellular signaling. It induces TIMD2 phosphorylation, inhibits angiogenesis, and collapses axonal growth cones. Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-His labeled tag.

Background

Semaphorin-4A (SEMA4A) serves as a cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1, playing a pivotal role in cell-cell signaling. This multifaceted protein is implicated in the regulation of glutamatergic and GABAergic synapse development, fostering inhibitory synapses in a PLXNB1-dependent manner and excitatory synapses in a PLXNB2-dependent manner. Additionally, SEMA4A contributes to adaptive immunity by priming antigen-specific T-cells, promoting the differentiation of Th1 T-helper cells, and facilitating the phosphorylation of TIMD2. Furthermore, SEMA4A exerts inhibitory effects on angiogenesis, induces axon growth cone collapse, and hinders axonal extension by providing localized signals that specify territories inaccessible for growing axons. Its intricate role is underscored by interactions with PLXNB1, PLXNB2, PLXNB3, PLXND1, and TIMD2, emphasizing its involvement in diverse cellular processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Human Semaphorin 4A is immobilized at 2.0 µg/mL(100 μL/well)can bind Human LILRB2/CD85d/ILT4(HY-P70168). The ED50 for this effect is 0.5264-0.5706 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA.When Recombinant Human Semaphorin 4A isimmobilized at 2 μg/mL(100 μL/well) can bind Recombinant Human LILRB2/CD85d/ILT4. The ED50 for this effect is 0.5706 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q9H3S1-1 (G33-H683)

  • Gene ID
    64218  [NCBI]
  • Molecular Construction
    • N-term
    • SEMA4A (G33-H683)
      Accession # Q9H3S1-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    SEMA4A; Semaphorin-4A; Prev. SEMAB; FLJ12287; CORD10; Sema B; SemB; RP35; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, (Semaphorin) 4A; SEMB; Semaphorin 4A; Semaphorin-B

  • AA Sequence

    GGGQGPMPRVRYYAGDERRALSFFHQKGLQDFDTLLLSGDGNTLYVGAREAILALDIQDPGVPRLKNMIPWPASDRKKSECAFKKKSNETQCFNFIRVLVSYNVTHLYTCGTFAFSPACTFIELQDSYLLPISEDKVMEGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDNFLRWLHHDASFVAAIPSTQVVYFFFEETASEFDFFERLHTSRVARVCKNDVGGEKLLQKKWTTFLKAQLLCTQPGQLPFNVIRHAVLLPADSPTAPHIYAVFTSQWQVGGTRSSAVCAFSLLDIERVFKGKYKELNKETSRWTTYRGPETNPRPGSCSVGPSSDKALTFMKDHFLMDEQVVGTPLLVKSGVEYTRLAVETAQGLDGHSHLVMYLGTTTGSLHKAVVSGDSSAHLVEEIQLFPDPEPVRNLQLAPTQGAVFVGFSGGVWRVPRANCSVYESCVDCVLARDPHCAWDPESRTCCLLSAPNLNSWKQDMERGNPEWACASGPMSRSLRPQSRPQIIKEVLAVPNSILELPCPHLSALASYYWSHGPAAVPEASSTVYNGSLLLIVQDGVGGLYQCWATENGFSYPVISYWVDSQDQTLALDPELAGIPREHVKVPLTRVSGGAALAAQQSYWPH

  • Molecular Weight

    Approximately 75-80 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween 80.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA.When Recombinant Human Semaphorin 4A isimmobilized at 2 μg/mL(100 μL/well) can bind Recombinant Human LILRB2/CD85d/ILT4. The ED50 for this effect is 0.5706 μg/mL.

  • Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Semaphorin-4A/SEMA4A Protein, Human (HEK293, His)
  • Experimental Validation Results for Semaphorin-4A/SEMA4A Protein, Human (HEK293, His)
  • Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) Product Image