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Semaphorin-4A/SEMA4A Protein, Human (HEK293, His)

Cat. No.: HY-P76056
COA Handling Instructions

Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1 and is essential for intercellular signaling. It induces TIMD2 phosphorylation, inhibits angiogenesis, and collapses axonal growth cones. Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-His labeled tag. The total length of Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) is 683 a.a., with molecular weight of 75-80 kDa.

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Description

Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1 and is essential for intercellular signaling. It induces TIMD2 phosphorylation, inhibits angiogenesis, and collapses axonal growth cones. Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-His labeled tag. The total length of Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) is 683 a.a., with molecular weight of 75-80 kDa.

Background

Semaphorin-4A (SEMA4A) serves as a cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1, playing a pivotal role in cell-cell signaling. This multifaceted protein is implicated in the regulation of glutamatergic and GABAergic synapse development, fostering inhibitory synapses in a PLXNB1-dependent manner and excitatory synapses in a PLXNB2-dependent manner. Additionally, SEMA4A contributes to adaptive immunity by priming antigen-specific T-cells, promoting the differentiation of Th1 T-helper cells, and facilitating the phosphorylation of TIMD2. Furthermore, SEMA4A exerts inhibitory effects on angiogenesis, induces axon growth cone collapse, and hinders axonal extension by providing localized signals that specify territories inaccessible for growing axons. Its intricate role is underscored by interactions with PLXNB1, PLXNB2, PLXNB3, PLXND1, and TIMD2, emphasizing its involvement in diverse cellular processes.

Species

Human

Source

HEK293

Tag

C-His

Accession

Q9H3S1 (M1-H683)

Gene ID
Molecular Construction
N-term
SEMA4A (M1-H683)
Accession # Q9H3S1
His
C-term
Synonyms
Semaphorin-4A; Semaphorin-B; Sema B; Sema4a; Semab; SemB
AA Sequence

MALPALGLDPWSLLGLFLFQLLQLLLPTTTAGGGGQGPMPRVRYYAGDERRALSFFHQKGLQDFDTLLLSGDGNTLYVGAREAILALDIQDPGVPRLKNMIPWPASDRKKSECAFKKKSNETQCFNFIRVLVSYNVTHLYTCGTFAFSPACTFIELQDSYLLPISEDKVMEGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDNFLRWLHHDASFVAAIPSTQVVYFFFEETASEFDFFERLHTSRVARVCKNDVGGEKLLQKKWTTFLKAQLLCTQPGQLPFNVIRHAVLLPADSPTAPHIYAVFTSQWQVGGTRSSAVCAFSLLDIERVFKGKYKELNKETSRWTTYRGPETNPRPGSCSVGPSSDKALTFMKDHFLMDEQVVGTPLLVKSGVEYTRLAVETAQGLDGHSHLVMYLGTTTGSLHKAVVSGDSSAHLVEEIQLFPDPEPVRNLQLAPTQGAVFVGFSGGVWRVPRANCSVYESCVDCVLARDPHCAWDPESRTCCLLSAPNLNSWKQDMERGNPEWACASGPMSRSLRPQSRPQIIKEVLAVPNSILELPCPHLSALASYYWSHGPAAVPEASSTVYNGSLLLIVQDGVGGLYQCWATENGFSYPVISYWVDSQDQTLALDPELAGIPREHVKVPLTRVSGGAALAAQQSYWPH

Molecular Weight

75-80 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Semaphorin-4A/SEMA4A Protein, Human (HEK293, His)
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HY-P76056
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