Semaphorin-4A/SEMA4A Protein, Human (HEK293, His)

Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1 and is essential for intercellular signaling. It induces TIMD2 phosphorylation, inhibits angiogenesis, and collapses axonal growth cones. Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-His labeled tag.

Semaphorin-4A (SEMA4A) serves as a cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1, playing a pivotal role in cell-cell signaling. This multifaceted protein is implicated in the regulation of glutamatergic and GABAergic synapse development, fostering inhibitory synapses in a PLXNB1-dependent manner and excitatory synapses in a PLXNB2-dependent manner. Additionally, SEMA4A contributes to adaptive immunity by priming antigen-specific T-cells, promoting the differentiation of Th1 T-helper cells, and facilitating the phosphorylation of TIMD2. Furthermore, SEMA4A exerts inhibitory effects on angiogenesis, induces axon growth cone collapse, and hinders axonal extension by providing localized signals that specify territories inaccessible for growing axons. Its intricate role is underscored by interactions with PLXNB1, PLXNB2, PLXNB3, PLXND1, and TIMD2, emphasizing its involvement in diverse cellular processes.

Measured by its binding ability in a functional ELISA. When Human Semaphorin 4A is immobilized at 2.0 µg/mL(100 μL/well)can bind Human LILRB2/CD85d/ILT4(HY-P70168). The ED₅₀ for this effect is 0.5264-0.5706 μg/mL.

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  Measured by its binding ability in a functional ELISA.When Recombinant Human Semaphorin 4A isimmobilized at 2 μg/mL(100 μL/well) can bind Recombinant Human LILRB2/CD85d/ILT4. The ED₅₀ for this effect is 0.5706 μg/mL.

Human

HEK293

C-His

Q9H3S1-1 (G33-H683)

64218  [NCBI]

Extracellular Domain

GGGQGPMPRVRYYAGDERRALSFFHQKGLQDFDTLLLSGDGNTLYVGAREAILALDIQDPGVPRLKNMIPWPASDRKKSECAFKKKSNETQCFNFIRVLVSYNVTHLYTCGTFAFSPACTFIELQDSYLLPISEDKVMEGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDNFLRWLHHDASFVAAIPSTQVVYFFFEETASEFDFFERLHTSRVARVCKNDVGGEKLLQKKWTTFLKAQLLCTQPGQLPFNVIRHAVLLPADSPTAPHIYAVFTSQWQVGGTRSSAVCAFSLLDIERVFKGKYKELNKETSRWTTYRGPETNPRPGSCSVGPSSDKALTFMKDHFLMDEQVVGTPLLVKSGVEYTRLAVETAQGLDGHSHLVMYLGTTTGSLHKAVVSGDSSAHLVEEIQLFPDPEPVRNLQLAPTQGAVFVGFSGGVWRVPRANCSVYESCVDCVLARDPHCAWDPESRTCCLLSAPNLNSWKQDMERGNPEWACASGPMSRSLRPQSRPQIIKEVLAVPNSILELPCPHLSALASYYWSHGPAAVPEASSTVYNGSLLLIVQDGVGGLYQCWATENGFSYPVISYWVDSQDQTLALDPELAGIPREHVKVPLTRVSGGAALAAQQSYWPH

Approximately 70-80 kDa due to the glycosylation.

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  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween 80.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.