Copy Link Print Information

Semaphorin-7A/SEMA7A Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

Semaphorin-7A (SEMA7A) plays a key role in integrin-mediated signaling, affecting multiple cellular functions, including cell migration and immune responses. SEMA7A promotes focal adhesion complex formation, activates PTK2/FAK1 and induces phosphorylation of MAPK1 and MAPK3. Semaphorin-7A/SEMA7A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-7A/SEMA7A protein, expressed by HEK293 , with C-8*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Human (HEK293, His)
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Human (HEK293, His)
  • Semaphorin-7A/SEMA7A Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Semaphorin-7A (SEMA7A) plays a key role in integrin-mediated signaling, affecting multiple cellular functions, including cell migration and immune responses. SEMA7A promotes focal adhesion complex formation, activates PTK2/FAK1 and induces phosphorylation of MAPK1 and MAPK3. Semaphorin-7A/SEMA7A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-7A/SEMA7A protein, expressed by HEK293 , with C-8*His labeled tag.

Background

The Semaphorin-7A (SEMA7A) protein assumes a crucial role in integrin-mediated signaling, exerting its influence on diverse cellular functions that include the regulation of cell migration and immune responses. SEMA7A actively promotes the formation of focal adhesion complexes, activating protein kinase PTK2/FAK1 and subsequently inducing the phosphorylation of MAPK1 and MAPK3. Furthermore, it plays a significant role in orchestrating the production of pro-inflammatory cytokines by monocytes and macrophages, thereby modulating inflammation and T-cell-mediated immune responses. In developmental contexts, SEMA7A is implicated in promoting axon growth in the embryonic olfactory bulb and facilitates attachment, spreading, and dendrite outgrowth in melanocytes. Its intricate molecular interactions involve associations with integrins ITGA1 and ITGB1, suggesting its involvement in mediating cell adhesion, and it interacts with PLXNC1, adding to the complexity of its regulatory role in diverse cellular processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human SEMA7A at 2 μg/mL can bind Anti- SEMA7A antibody. The ED50 for this effect is 13.27 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human SEMA7A at 2 μg/mL can bind Anti- SEMA7A antibody. The ED50 for this effect is 13.27 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-8*His
  • Accession

    O75326-1 (Q45-A648)

  • Gene ID
    8482  [NCBI]
  • Molecular Construction
    • N-term
    • SEMA7A (Q45-A648)
      Accession # O75326-1
    • 8*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    SEMA7A; CDw108; Prev. SEMAL; Sema Domain, Immunoglobulin Domain (Ig), And GPI Membrane Anchor, (Semaphorin) 7A; CD108; Sema Domain, Immunoglobulin Domain (Ig), And GPI Membrane Anchor, 7A; Semaphorin-7A; Semaphorin 7A (John Milton Hagen Blood Group); Sema

  • AA Sequence

    QGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALA

  • Predicted Molecular Mass

    69.5 kDa

  • Molecular Weight

    Approximately 70-77 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human SEMA7A at 2 μg/mL can bind Anti- SEMA7A antibody. The ED50 for this effect is 13.27 ng/mL.

  • Semaphorin-7A/SEMA7A Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Human (HEK293, His)
  • Experimental Validation Results for Semaphorin-7A/SEMA7A Protein, Human (HEK293, His)
  • Semaphorin-7A/SEMA7A Protein, Human (HEK293, His) Product Image