Serpin A5 Protein, Mouse (sf9, His)

Serpin A5 protein is a heparin-dependent serine protease inhibitor that controls proteolytic activity in body fluids. It binds irreversibly to the serine activation site, inactivating serine proteases and regulating intravascular and extravascular proteolysis. Serpin A5 Protein, Mouse (sf9, His) is the recombinant mouse-derived Serpin A5 protein, expressed by sf9 insect cells , with N-10*His labeled tag.

Serpin A5 Protein, a heparin-dependent serine protease inhibitor, intricately governs proteolytic activities in body fluids and secretions. This multifaceted protein exerts its function by irreversibly binding to the serine activation site of serine proteases, thereby inactivating them. With a role in the regulation of both intravascular and extravascular proteolysis, Serpin A5 plays a crucial hemostatic role in blood plasma. Acting as a procoagulant and pro-inflammatory factor, it inhibits activated protein C factor and the generation of activated protein C factor by the thrombin/thrombomodulin complex. Simultaneously, it acts as an anticoagulant by inhibiting factors like prothrombin, factor XI, factor Xa, plasma kallikrein, and fibrinolytic enzymes. In seminal plasma, Serpin A5 targets serine proteases in the reproductive system, indirectly protecting male genital tract components from excessive acrosin release. It further regulates sperm motility and fertilization by inhibiting various enzymes. In urine, the protein inhibits plasminogen activator and kallikrein activities, while also inactivating membrane-anchored serine proteases involved in tumor cell invasion and metastasis. Additionally, Serpin A5 may serve a non-inhibitory role as a hydrophobic hormone carrier in seminal plasma and urine through its binding to retinoic acid. Its inhibitory potency is significantly enhanced in the presence of glycosaminoglycans, heparin, thrombomodulin, and phospholipid vesicles.

Mouse

Sf9 insect cells

N-10*His

P70458 (S25-P405)

268591  [NCBI]

Full Length of Mature Protein

SKKKKAKESSVGAVGPPSSKDFAFRLYRALVSESPGQNVFFSPLSVSMSLGMLSLGAGLKTKTQILDGLGLSLQQGQEDKLHKGFQQLLQRFRQPSDGLQLSLGSALFKDPAVHIRDDFLSAMKTLYMSDTFSTNFGNPEIAKKQINNYVAKQTKGKIVDFIKDLDSTHVMIVVNYIFFKAKWQTAFSETNTHKMDFHVTPKRTTQVPMMNREDGYSYYLDQNISCTVVGIPYQGNAIALFILPSEGKMKQVEDGLDERTLRNWLKMFTKRRLDLYLPKFSIEATYKLENVLPKLGIQDVFTTHADLSGITDHTNIKLSEMVHKSMMEVEESGTTAAAITGAIFTFRSARPSSLKIEFTRPFLLTLMEDSHILFVGKVTRP

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.