Serpin B2 Protein, Human
Based on 1 Customer Validation
Serpin B2 inhibits urokinase-type plasminogen activator and contributes to fibrinolytic regulation. Monocyte-derived plasminogen activator inhibitor 2 (PAI-2) differs from endothelial cell-derived PAI-1, highlighting different cellular origins. Serpin B2 Protein, Human is the recombinant human-derived Serpin B2 protein, expressed by E. coli , with tag free. The total length of Serpin B2 Protein, Human is 415 a.a., with molecular weight of ~46.6 kDa.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Serpin B2 inhibits urokinase-type plasminogen activator and contributes to fibrinolytic regulation. Monocyte-derived plasminogen activator inhibitor 2 (PAI-2) differs from endothelial cell-derived PAI-1, highlighting different cellular origins. Serpin B2 Protein, Human is the recombinant human-derived Serpin B2 protein, expressed by E. coli , with tag free. The total length of Serpin B2 Protein, Human is 415 a.a., with molecular weight of ~46.6 kDa.
Serpin B2 functions as an inhibitor of urokinase-type plasminogen activator, contributing to the regulation of fibrinolysis. Notably, the monocyte-derived form of plasminogen activator inhibitor-2 (PAI-2) differs from the endothelial cell-derived PAI-1, emphasizing the diverse cellular origins and roles within the intricate network of protease regulation. Serpin B2 is intricately involved in modulating the activity of urokinase-type plasminogen activator, reflecting its significance in the dynamic interplay of proteolytic processes. Additionally, Serpin B2 engages in interactions with PSMB1, possibly participating in complex cellular pathways that extend beyond its classical role as a plasminogen activator inhibitor.
Measured by its ability to inhibit uPA ( HY-P71050) cleavage of a colorimetric peptide substrate, Z-Gly-Gly-Arg-AMC (Z-GGR-AMC) (HY-P0019) that incubate at room temperature for 15 minutes. The IC50 value is 14.16 nM.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
P05120 (M1-P415)
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Molecular Construction
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N-term
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Serpin B2 (M1-P415)
Accession # P05120 -
C-term
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Protein Length
Full Length
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Synonyms
SERPINB2; Placental Plasminogen Activator Inhibitor; Prev. PLANH2; Plasminogen-Activator Inhibitor; Prev. PAI2; Monocyte Arg-Serpin; PAI-2; Urokinase Inhibitor; HsT1201; Serpin B2; Serine (Or Cysteine) Proteinase Inhibitor, Clade B (Ovalbumin), Member 2;
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AA Sequence
MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKINSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP
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Predicted Molecular Mass
46.6 kDa
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Molecular Weight
Approximately 46 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM Cysteine, pH 8.0, 5% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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