Serpin B2 Protein, Human

Serpin B2 inhibits urokinase-type plasminogen activator and contributes to fibrinolytic regulation. Monocyte-derived plasminogen activator inhibitor 2 (PAI-2) differs from endothelial cell-derived PAI-1, highlighting different cellular origins. Serpin B2 Protein, Human is the recombinant human-derived Serpin B2 protein, expressed by E. coli , with tag free. The total length of Serpin B2 Protein, Human is 415 a.a., with molecular weight of ~46.6 kDa.

Serpin B2 functions as an inhibitor of urokinase-type plasminogen activator, contributing to the regulation of fibrinolysis. Notably, the monocyte-derived form of plasminogen activator inhibitor-2 (PAI-2) differs from the endothelial cell-derived PAI-1, emphasizing the diverse cellular origins and roles within the intricate network of protease regulation. Serpin B2 is intricately involved in modulating the activity of urokinase-type plasminogen activator, reflecting its significance in the dynamic interplay of proteolytic processes. Additionally, Serpin B2 engages in interactions with PSMB1, possibly participating in complex cellular pathways that extend beyond its classical role as a plasminogen activator inhibitor.

Measured by its ability to inhibit uPA ( HY-P71050) cleavage of a colorimetric peptide substrate, Z-Gly-Gly-Arg-AMC (Z-GGR-AMC) (HY-P0019) that incubate at room temperature for 15 minutes. The IC₅₀ value is 14.16 nM.

Human

E. coli

Tag Free

P05120 (M1-P415)

5055  [NCBI]

Full Length

MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKINSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP

Approximately 46 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM Cysteine, pH 8.0, 5% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.