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Serpin F1 Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

Serpin F1 is a neurotrophin that significantly induces extensive neuronal differentiation of retinoblastoma cells and exhibits potent anti-angiogenic effects.Its unique conformation lacks the S (stress) to R (relaxation) transition typical of active serpins, resulting in a lack of serpin activity.Serpin F1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin F1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Serpin F1 is a neurotrophin that significantly induces extensive neuronal differentiation of retinoblastoma cells and exhibits potent anti-angiogenic effects.Its unique conformation lacks the S (stress) to R (relaxation) transition typical of active serpins, resulting in a lack of serpin activity.Serpin F1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin F1 protein, expressed by HEK293 , with C-His labeled tag.

Background

Serpin F1, a neurotrophic protein, emerges as a key inducer of extensive neuronal differentiation in retinoblastoma cells. Notably, it exerts a potent inhibitory effect on angiogenesis. Distinguished by its unique conformational characteristics, Serpin F1 does not undergo the S (stressed) to R (relaxed) transition typical of active serpins, resulting in the absence of serine protease inhibitory activity. Furthermore, its interaction with PNPLA2 serves to stimulate the phospholipase A2 activity of PNPLA2, underscoring the multifaceted roles of Serpin F1 in cellular processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized mouse SERPINF1-His at 10 μg/mL (100 μL/well) can bind human GST-CSNK2A1 with a linear range of 0.31-2.5 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    P97298 (Q20-T417)

  • Gene ID
    20317  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin F1 (Q20-T417)
      Accession # P97298
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SERPINF1; Testis Tissue Sperm-Binding Protein Li 70n; Prev. PEDF; Serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1; EPC-1; Proliferation-Inducing Protein 35; Pigment Epithelium-Derived Factor; Serpin F

  • AA Sequence

    QNVPSSSEGSPVPDSTGEPVEEEDPFFKVPVNKLAAAVSNFGYDLYRLRSSASPTGNVLLSPLSVATALSALSLGAEHRTESVIHRALYYDLITNPDIHSTYKELLASVTAPEKNLKSASRIVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRVDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWVTKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLTMIEESLTSEFIHDIDRELKTIQAVLTVPKLKLSFEGELTKSLQDMKLQSLFESPDFSKITGKPVKLTQVEHRAAFEWNEEGAGSSPSPGLQPVRLTFPLDYHLNQPFLFVLRDTDTGALLFIGRILDPSST

  • Predicted Molecular Mass

    45.8 kDa

  • Molecular Weight

    Approximately 60-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
2.Lyophilized from a 0.22 μm filtered solution of 25 mM MES, 150 mM NaCl, 0.02% Tween 20, pH 5.5, 5% trehalose, 5% mannitol, 0.01% Tween 80.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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