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Serpin H1 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

Serpin H1 protein exhibits a specific affinity for collagen, indicating its role as a chaperone in the biosynthetic pathway of collagen. This suggests its potential contribution to collagen synthesis, emphasizing its importance in maintaining tissue integrity and modulating cellular responses related to collagen metabolism. Serpin H1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin H1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Serpin H1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Serpin H1 Protein, Human (HEK293, His)
  • Serpin H1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Serpin H1 protein exhibits a specific affinity for collagen, indicating its role as a chaperone in the biosynthetic pathway of collagen. This suggests its potential contribution to collagen synthesis, emphasizing its importance in maintaining tissue integrity and modulating cellular responses related to collagen metabolism. Serpin H1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin H1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The Serpin H1 Protein demonstrates a specific affinity for collagen, indicating its role in selectively binding to this extracellular matrix protein. This interaction suggests that Serpin H1 may play a functional role as a chaperone in the biosynthetic pathway of collagen, potentially contributing to the intricate process of collagen synthesis. By virtue of its specific binding to collagen, Serpin H1 likely participates in regulating collagen-related biological processes, emphasizing its potential importance in maintaining the structural integrity of tissues and modulating cellular responses associated with collagen metabolism.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human Serpin H1 at 2 μg/mL can bind Anti-Serpin H1 antibody. The ED50 for this effect is 54.55 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human Serpin H1 at 2 μg/mL can bind Anti-Serpin H1 antibody. The ED50 for this effect is 54.55 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P50454 (A19-L418)

  • Gene ID
    871  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin H1 (A19-L418)
      Accession # P50454
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SERPINH1; Collagen-Binding Protein; Prev. SERPINH2; Colligin; Prev. CBP1; AsTP3; Prev. CBP2; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2, (Collagen-Binding Protein 2); HSP47; Serine (Or Cysteine) Proteinase Inhibit

  • AA Sequence

    AEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

  • Predicted Molecular Mass

    45.3 kDa

  • Molecular Weight

    Approximately 48-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human Serpin H1 at 2 μg/mL can bind Anti-Serpin H1 antibody. The ED50 for this effect is 54.55 ng/mL.

  • Serpin H1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Serpin H1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Serpin H1 Protein, Human (HEK293, His)
  • Serpin H1 Protein, Human (HEK293, His) Product Image