Siglec-9 Protein, Cynomolgus (HEK293, His)
Siglec-9 Protein is a member of the Siglec cell surface immunoglobulin family. Siglec-9 is highly expressed on human neutrophils and monocytes and low on natural killer cells, and sub-populations of T and B lymphocytes. Siglec-9 induces human neutrophil apoptosis and autophagy-like cell death and also inhibits tumor activity. Siglec-9 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Siglec-9 protein, expressed by HEK293 , with C-His labeled tag. The total length of Siglec-9 Protein, Cynomolgus (HEK293, His) is 331 a.a., with molecular weight of 55-70 kDa.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Siglec-9 Protein is a member of the Siglec cell surface immunoglobulin family. Siglec-9 is highly expressed on human neutrophils and monocytes and low on natural killer cells, and sub-populations of T and B lymphocytes. Siglec-9 induces human neutrophil apoptosis and autophagy-like cell death and also inhibits tumor activity[1][2][3]. Siglec-9 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Siglec-9 protein, expressed by HEK293 , with C-His labeled tag. The total length of Siglec-9 Protein, Cynomolgus (HEK293, His) is 331 a.a., with molecular weight of 55-70 kDa.
Background
Sialic acid-binding immunoglobulin-like lectin (Siglec-9) is a member of the Siglec cell surface immunoglobulin family. Siglec-9 is highly expressed on human neutrophils and monocytes and low on natural killer cells, and sub-populations of T and B lymphocytes. Ligation of Siglec-9 by chemical compounds or synthetic ligands induced apoptosis and autophagic-like cell death in human neutrophils. Siglec-9 binds to sialic acid and transduces apoptotic and nonapoptotic death signals to neutrophils. Siglec-9 is an immune-checkpoint molecule on macrophages that can be targeted to enhance anti-PD-1/PD-L1 therapeutic efficacy for GBM treatment. Pro-tumorigenic activity can be suppressed by blocking Siglec-9, which unleashes antitumor functions and induces robust tumor-eliminating activity. Targeting Siglec-9 could be beneficial for the treatment of COPD, asthma, fibrosis, and related chronic inflammatory lung diseases[1][2][3].
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-His
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Accession
XP_005590140 (Q20-G350)
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Molecular Construction
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N-term
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Siglec-9 (Q20-G350)
Accession # XP_005590140 -
His
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C-term
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Protein Length
Partial
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Synonyms
SIGLEC9; CDw329; Sialic Acid Binding Ig Like Lectin 9; Sialic Acid Binding Ig-Like Lectin 9; CD329; CD329 Antigen; Sialic Acid-Binding Ig-Like Lectin 9; OBBP-LIKE; Protein FOAP-9; FOAP-9; Siglec-9
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AA Sequence
QRNNQKNYPLMMQESVTVQQGLCVHVLCSFSYPWYGWISSDPVHGYWFRAGARTDRDAPVATNNPARAVREDTRDRFHLLGDPQTKNCTLSIRDARSSDAGKYFFRVETGKTKWNYKYAPLSVHVTALTHRPNILIPGTLESGCPRNLTCSVPWACEQGTAPMISWMGTSVSPLDPSTTRSSVLTLIPQPQDHGTSLTCQVTFPGASVTTNKTIHLNVSYPPQNLTMTVFQGNDTVSIVLGNGSSVSVPEGPSLRLVCAVDSNPPARLSLSWGGLTLCPSQPSNPGVLELPRVHLRDEEEFTCRAQNLLGSQQVSLNVSLQSKATSGLTQG
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Predicted Molecular Mass
38 kDa
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Molecular Weight
Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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