SIRP beta 1 Protein, Human (HEK293, hFc)

SIRP beta 1 protein negatively regulates receptor tyrosine kinase signaling and recruits SYK for myeloid cell activation. It interacts with TYROBP as a homodimer, facilitating SYK recruitment. This intricate molecular interplay is crucial for modulating myeloid cell activation and regulating cellular signaling. SIRP beta 1 occupies a central position in protein interactions that contribute to its functional role. SIRP beta 1 Protein, Human (HEK293, hFc) is the recombinant human-derived SIRP beta 1 protein, expressed by HEK293, with C-hFc labeled tag.

SIRP beta 1 protein, an immunoglobulin-like cell surface receptor, plays a vital role in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Additionally, it participates in the recruitment of the tyrosine kinase SYK, triggering the activation of myeloid cells when associated with TYROBP. Existing as a homodimer with disulfide linkages, SIRP beta 1 interacts specifically with TYROBP, leading to the recruitment of SYK. This intricate molecular interplay positions SIRP beta 1 at the nexus of cellular signaling regulation, highlighting its significance in modulating myeloid cell activation and the intricate network of protein interactions that contribute to its functional role.

Human

HEK293

C-hFc

O00241-1 (E30-L371)

10326

Extracellular Domain

EDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHEAALAPTAPL

Approximately 70-80 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.