SMOC1 Protein, Human (408a.a, HEK293, His)
Based on 1 Customer Validation
SMOC1 Protein is a matricellular protein that interferes with growth factor receptor signalling. SMOC1 Protein plays essential roles in both eye and limb development and is a regulator of osteoblast differentiation. SMOC1 Protein, Human (408a.a, HEK293, His) is the recombinant human-derived SMOC1 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
SMOC1 Protein is a matricellular protein that interferes with growth factor receptor signalling. SMOC1 Protein plays essential roles in both eye and limb development and is a regulator of osteoblast differentiation. SMOC1 Protein, Human (408a.a, HEK293, His) is the recombinant human-derived SMOC1 protein, expressed by HEK293 , with C-His labeled tag.
Background
SMOC1 Protein is a matricellular protein that interferes with growth factor receptor signalling. SMOC1 Protein plays essential roles in both eye and limb development and is a regulator of osteoblast differentiation. SMOC1 encodes a multi-domain secreted protein that may have a critical role in ocular and limb development. Mutations in this gene are associated with microphthalmia and limb anomalies[1][2].
Verified Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8067 μg/mL, corresponding to a specific activity is 1239.618 units/mg.
MCE Validation Data
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
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Species Human
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Source HEK293
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Tag C-8*His
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Accession
Q9H4F8-1 (H27-V434)
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Gene ID64093
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Molecular Construction
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N-term
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SMOC1 (H27-V434)
Accession # Q9H4F8-1 -
8*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
SMOC1; Secreted Modular Calcium-Binding Protein 1; SPARC Related Modular Calcium Binding 1; SMOC-1; SPARC-Related Modular Calcium-Binding Protein 1; OAS
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AA Sequence
HRTTGPRFLISDRDPQCNLHCSRTQPKPICASDGRSYESMCEYQRAKCRDPTLGVVHRGRCKDAGQSKCRLERAQALEQAKKPQEAVFVPECGEDGSFTQVQCHTYTGYCWCVTPDGKPISGSSVQNKTPVCSGSVTDKPLSQGNSGRKDDGSKPTPTMETQPVFDGDEITAPTLWIKHLVIKDSKLNNTNIRNSEKVYSCDQERQSALEEAQQNPREGIVIPECAPGGLYKPVQCHQSTGYCWCVLVDTGRPLPGTSTRYVMPSCESDARAKTTEADDPFKDRELPGCPEGKKMEFITSLLDALTTDMVQAINSAAPTGGGRFSEPDPSHTLEERVVHWYFSQLDSNSSNDINKREMKPFKRYVKKKAKPKKCARRFTDYCDLNKDKVISLPELKGCLGVSKEGRLV
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Predicted Molecular Mass
46.4 kDa
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Molecular Weight
Approximately 60-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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