SNAP25 Protein, Human (His)

SNAP25 is a key t-SNARE in neurotransmitter release, regulating synaptic function and plasma membrane recycling. It cooperates with CENPF to affect vesicle docking and membrane fusion. SNAP25 Protein, Human (His) is the recombinant human-derived SNAP25 protein, expressed by E. coli , with N-His labeled tag.

SNAP25, a t-SNARE integral to the molecular orchestration of neurotransmitter release, assumes a pivotal role in synaptic function within specific neuronal systems. Forming associations with proteins involved in vesicle docking and membrane fusion, SNAP25 intricately regulates plasma membrane recycling through interaction with CENPF. Its modulatory influence extends to the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. As part of the SNARE core complex, comprised of SNAP25, VAMP2, and STX1A, it constitutes the fundamental catalytic machinery for complex neurotransmitter release. Recruitment to the SNARE complex is facilitated by the binding of STX1A to STXBP1. Additionally, SNAP25 participates in diverse complexes involving SYT1, SV2B, syntaxin-1, CPLX1, and BLOC1S6. Interactions with HSC70, SYT9, DNAJC5, CENPF, EQTN, HGS, KCNB1, OTOF, RIMS1, SNAPIN, STXBP6, TRIM9, ZDHHC13, ZDHHC17, PLCL1, PRRT2, SNCA, PRPH2, ROM1, and STX3 further underscore its intricate molecular engagements, highlighting its multifaceted contributions to cellular processes and signaling pathways.

Human

E. coli

N-His

P60880 (M1-G206)

6616  [NCBI]

Full Length

MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG

Approximately 28 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.