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SR-BI/SCARB1 Protein, Human (HEK293, His)

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The SR-BI/SCARB1 protein is a multifunctional receptor that binds a variety of ligands, including phospholipids, cholesteryl esters, lipoproteins, phosphatidylserine, and apoptotic cells. As an HDL receptor, it crucially mediates selective cholesterol ether uptake and HDL-dependent cholesterol efflux. SR-BI/SCARB1 Protein, Human (HEK293, His) is the recombinant human-derived SR-BI/SCARB1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The SR-BI/SCARB1 protein is a multifunctional receptor that binds a variety of ligands, including phospholipids, cholesteryl esters, lipoproteins, phosphatidylserine, and apoptotic cells. As an HDL receptor, it crucially mediates selective cholesterol ether uptake and HDL-dependent cholesterol efflux. SR-BI/SCARB1 Protein, Human (HEK293, His) is the recombinant human-derived SR-BI/SCARB1 protein, expressed by HEK293 , with C-His labeled tag.

Background

SR-BI/SCARB1 Protein, functioning as a versatile receptor, binds to various ligands, including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and apoptotic cells. As a receptor for HDL, it plays a crucial role in the selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux. Additionally, SR-BI facilitates the exchange of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins, albeit less efficiently than with HDL. Its involvement in phagocytosis of apoptotic cells, mediated by its phosphatidylserine binding activity, adds another layer to its functional repertoire. In the context of microbial infection, SR-BI acts as a receptor for the hepatitis C virus in hepatocytes, facilitating its cellular entry independently of the viral isolate genotype. The diverse ligand interactions and roles in cholesterol homeostasis, apoptotic cell clearance, and viral infection highlight SR-BI/SCARB1 as a multifunctional player in cellular processes.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q8WTV0-1/NP_001076428.1 (P33-Y443)

  • Gene ID
    949  [NCBI]
  • Molecular Construction
    • N-term
    • SR-BI (P33-Y443)
      Accession # Q8WTV0
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    SCARB1; CD36 And LIMPII Analogous 1; Prev. CD36L1; Scavenger Receptor Class B, Member 1; SR-BI; Scavenger Receptor Class B Type III; CLA-1; CD36 Antigen-Like 1; SRB1; CD36 Antigen; CLA1; HDLQTL6; CD36 Antigen (Collagen Type I Receptor, Thrombospondin Rece

  • AA Sequence

    PSLIKQQVLKNVRIDPSSLSFNMWKEIPIPFYLSVYFFDVMNPSEILKGEKPQVRERGPYVYREFRHKSNITFNNNDTVSFLEYRTFQFQPSKSHGSESDYIVMPNILVLGAAVMMENKPMTLKLIMTLAFTTLGERAFMNRTVGEIMWGYKDPLVNLINKYFPGMFPFKDKFGLFAELNNSDSGLFTVFTGVQNISRIHLVDKWNGLSKVDFWHSDQCNMINGTSGQMWPPFMTPESSLEFYSPEACRSMKLMYKESGVFEGIPTYRFVAPKTLFANGSIYPPNEGFCPCLESGIQNVSTCRFSAPLFLSHPHFLNADPVLAEAVTGLHPNQEAHSLFLDIHPVTGIPMNCSVKLQLSLYMKSVAGIGQTGKIEPVVLPLLWFAESGAMEGETLHTFYTQLVLMPKVMHY

  • Predicted Molecular Mass

    47.8 kDa

  • Molecular Weight

    Approximately 70-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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