Streptavidin Protein
Based on 1 Customer Validation
Streptavidin Protein is the recombinant Streptavidin, expressed by E. coli , with tag Free labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Streptavidin Protein is the recombinant Streptavidin, expressed by E. coli , with tag Free labeled tag.
Streptavidin forms a strong, non-covalent, specific complex with biotin. Each subunit of streptavidin can bind one biotin molecule, forming a homotetramer in structure. Streptavidin can be used in a variety of molecular and cellular processes that require specific binding to biotinylated molecules.
>15 U/mg protein, 1 U is defined as the quantity of protein that binds 1 microgramme.
Technical Parameters
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Species Others
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Source E. coli
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Tag Tag Free
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Accession
Sequence information is not available or disclosed for this product.
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Gene ID/
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Synonyms
Streptavidin
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AA Sequence
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Predicted Molecular Mass
13.5 kDa
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Molecular Weight
Approximately 14 kDa, based on SDS-PAGE under reducing conditions,112 kDa based on SDS-PAGE under non-reduced conditions,due to the glycosylation.
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Structure/Form
Octamer
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 10 mM PB, pH 6.5-7.0.
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It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (233 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)