1. Recombinant Proteins
  2. CAR-T Related Proteins CD Antigens
  3. CD138/Syndecan-1 B Cell CD Proteins Stem Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. Syndecan-1/CD138 Protein, Mouse (HEK293, C-His)

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Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Tamaño Precio Stock Cantidad
10 μg En stock
50 μg En stock
100 μg En stock
> 100 μg   Obtener un presupuesto  

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  • Actividad biológica

  • Technical Parameters

  • Properties

  • Descripciòn

Actividad biológica

Measured by the ability of the immobilized protein to enhance the adhesion of Saos‑2 human osteosarcoma cells to human Fibronectin. The ED50 for this effect is 1.533 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 652.316 units/mg.

Species

Mouse

Source

HEK293

Tag

C-10*His

Accession

P18828-1/NP_035649.1 (Q23-E252)

Gene ID

20969

Molecular Construction
N-term
Syndecan-1/CD138 (Q23-E252)
Accession # P18828-1/NP_035649.1
10*His
C-term
Protein Length

Extracellular Domain

Synonyms
Syndecan-1; SYND1; CD138; SDC1; SDC
AA Sequence

QIVAVNVPPEDQDGSGDDSDNFSGSGTGALPDTLSRQTPSTWKDVWLLTATPTAPEPTSSNTETAFTSVLPAGEKPEEGEPVLHVEAEPGFTARDKEKEVTTRPRETVQLPITQRASTVRVTTAQAAVTSHPHGGMQPGLHETSAPTAPGQPDHQPPRVEGGGTSVIKEVVEDGTANQLPAGEGSGEQDFTFETSGENTAVAAVEPGLRNQPPVDEGATGASQSLLDRKE

Predicted Molecular Mass
25.3 kDa
Peso molecular

Approximately 43-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Pureza

≥ 95%, as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Envío

Room temperature in continental US; may vary elsewhere.

Documentación
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Syndecan-1/CD138 Protein, Mouse (HEK293, C-His)

 

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Inquiry Information

Nombre del producto:
Syndecan-1/CD138 Protein, Mouse (HEK293, C-His)
Cat. No.:
HY-P76101A
Cantidad:
MCE Japan Authorized Agent: