Syntaxin-BP1 Protein, Human (sf9, His-GST)

Syntaxin-BP1 protein critically regulates synaptic vesicle docking and fusion, binds GTP-binding proteins and ensures efficient neurotransmission. It interacts with syntaxin in a 1:1 ratio and shows specificity for syntaxin 1, 2, and 3 (excluding syntaxin 4). Syntaxin-BP1 Protein, Human (sf9, His-GST) is the recombinant human-derived Syntaxin-BP1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Syntaxin-BP1 Protein plays a crucial role in the regulation of synaptic vesicle docking and fusion by interacting with GTP-binding proteins, and it is essential for efficient neurotransmission. This protein binds to syntaxin, a key component of the synaptic vesicle fusion machinery, likely in a 1:1 ratio, and demonstrates interaction specificity with syntaxins 1, 2, and 3, excluding syntaxin 4. Syntaxin-BP1 is actively involved in neurotransmitter release from neurons by interacting with SNARE complex component STX1A, facilitating the assembly of the SNARE complex at synaptic membranes. This interaction recruits SNARE complex components SNAP25 and VAMP2, contributing to the mediation of neurotransmitter release. Moreover, Syntaxin-BP1 engages in interactions with alpha-synuclein/SNCA, exerting control over SNCA self-replicating aggregation. Additionally, its interaction with RAB3A promotes RAB3A dissociation from the vesicle membrane, further emphasizing its multifaceted role in synaptic processes. The protein also interacts with CABP5, highlighting its involvement in diverse molecular interactions within the neuronal environment.

Human

Sf9 insect cells

N-His;N-GST

P61764 (M1-S594)

6812  [NCBI]

Full Length

MAPIGLKAVVGEKIMHDVIKKVKKKGEWKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDSCPDALFNELVKSRAAKVIKTLTEINIAFLPYESQVYSLDSADSFQSFYSPHKAQMKNPILERLAEQIATLCATLKEYPAVRYRGEYKDNALLAQLIQDKLDAYKADDPTMGEGPDKARSQLLILDRGFDPSSPVLHELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFSSSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHLHLAEDCMKHYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKNGITEENLNKLIQHAQIPPEDSEIITNMAHLGVPIVTDSTLRRRSKPERKERISEQTYQLSRWTPIIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLNKTDEEISS

Approximately 80 kDa, based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.