T Plastin/PLS3 Protein, Human (His)
T Plastin/PLS3 Protein, an actin-bundling protein, resides in cellular structures like intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. Operating as a monomer, T Plastin/PLS3 implies a versatile role in organizing actin within diverse cellular contexts. T Plastin/PLS3 Protein, Human (His) is the recombinant human-derived T Plastin/PLS3 protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
T Plastin/PLS3 Protein, an actin-bundling protein, resides in cellular structures like intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. Operating as a monomer, T Plastin/PLS3 implies a versatile role in organizing actin within diverse cellular contexts. T Plastin/PLS3 Protein, Human (His) is the recombinant human-derived T Plastin/PLS3 protein, expressed by E. coli , with N-His labeled tag.
Background
T Plastin/PLS3, an actin-bundling protein, is located in various cellular structures such as intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It is implicated in potential regulatory functions related to bone development. Operating as a monomer, T Plastin/PLS3 suggests a versatile role in actin organization within distinct cellular contexts.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
P13797 (G102-N375)
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Molecular Construction
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N-term
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His
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PLS3 (G102-N375)
Accession # P13797 -
C-term
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Protein Length
Partial
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Synonyms
PLS3; T Fimbrin; Plastin 3; T Plastin; T-Plastin; T-Fimbrin; Plastin-3; BMND18; Plastin 3 (T Isoform); DIH5; PLS3 Protein
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AA Sequence
GICALGGTSELSSEGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTDDLFKAVGDGIVLCKMINLSVPDTIDERAINKKKLTPFIIQENLNLALNSASAIGCHVVNIGAEDLRAGKPHLVLGLLWQIIKIGLFADIELSRNEALAALLRDGETLEELMKLSPEELLLRWANFHLENSGWQKINNFSADIKDSKAYFHLLNQIAPKGQKEGEPRIDINMSGFNETDDLKRAESMLQQADKLGCRQFVTPADVVSGNPKLNLAFVANLFN
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Predicted Molecular Mass
32.2 kDa
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Molecular Weight
Approximately 30-34 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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