T4 ligase Protein, T4 phage (His)
The T4 ligase protein is expressed during the early stages of lytic development and serves as a DNA ligase involved in various aspects of T4 phage biology. It plays a crucial role in T4 DNA synthesis, promoting the formation of phosphodiester bonds to join DNA fragments. T4 ligase Protein, T4 phage (His) is the recombinant T4 ligase protein, expressed by E. coli , with N-6*His labeled tag. T4 ligase Protein, T4 phage (His), has molecular weight of ~55.3 kDa.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The T4 ligase protein is expressed during the early stages of lytic development and serves as a DNA ligase involved in various aspects of T4 phage biology. It plays a crucial role in T4 DNA synthesis, promoting the formation of phosphodiester bonds to join DNA fragments. T4 ligase Protein, T4 phage (His) is the recombinant T4 ligase protein, expressed by E. coli , with N-6*His labeled tag. T4 ligase Protein, T4 phage (His), has molecular weight of ~55.3 kDa.
Background
T4 ligase is a DNA ligase enzyme expressed during the early stages of lytic development in the T4 bacteriophage life cycle. This enzyme is integral to T4 DNA synthesis and genetic recombination processes, participating in the joining of DNA fragments. Beyond its role in DNA synthesis and recombination, T4 ligase is implicated in T4 DNA repair mechanisms, suggesting its involvement in maintaining the integrity of the viral genome. The multifunctional nature of T4 ligase underscores its significance in the intricate processes associated with T4 bacteriophage development and highlights its role in ensuring the fidelity and stability of the viral DNA.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-6*His
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Accession
P00970 Sequence information is not available or disclosed for this product.
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Gene ID1258680 [NCBI]
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Molecular Construction
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N-term
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6*His
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T4 ligase
Accession # P00970 -
C-term
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Synonyms
DNA ligase; EC:6.5.1.1; Polydeoxyribonucleotide synthase [ATP]; 30
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AA Sequence
MILKILNEIASIGSTKQKQAILEKNKDNELLKRVYRLTYSRGLQYYIKKWPKPGIATQSFGMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEVLRRVMMRDLECGASVSIANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGARCFAEVRGDELDDVRLLSRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHEQVKKEPEGLDFLFDAYPENSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQVWDYVPLVEIYSLPAFRLKYDVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYIDQGLEGIILKNIDGLWENARSKNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECGKIKVNAGSGLKDKAGVKSHELDRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFLPIAIRLREDKTKANTFEDVFGDFHEVTGL
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Molecular Weight
Approximately 55 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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