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TEM8/ANTXR1 Protein, Human (HEK293, His)

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The TEM8/ANTXR1 protein is multifunctional in cell attachment and migration, interacting with extracellular matrix proteins and the actin cytoskeleton. This binding mediates cell adhesion to type 1 collagen and gelatin, promoting actin cytoskeletal reorganization and cell spreading, which is critical for cell structure and movement. TEM8/ANTXR1 Protein, Human (HEK293, His) is the recombinant human-derived TEM8/ANTXR1 protein, expressed by HEK293, with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • TEM8/ANTXR1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The TEM8/ANTXR1 protein is multifunctional in cell attachment and migration, interacting with extracellular matrix proteins and the actin cytoskeleton. This binding mediates cell adhesion to type 1 collagen and gelatin, promoting actin cytoskeletal reorganization and cell spreading, which is critical for cell structure and movement. TEM8/ANTXR1 Protein, Human (HEK293, His) is the recombinant human-derived TEM8/ANTXR1 protein, expressed by HEK293, with C-6*His labeled tag.

Background

TEM8/ANTXR1, a multifaceted protein, actively participates in cell attachment and migration. It engages with both extracellular matrix proteins and the actin cytoskeleton, mediating cell adhesion to type 1 collagen and gelatin. This interaction facilitates the reorganization of the actin cytoskeleton and promotes cell spreading, underscoring its crucial role in cellular architecture and motility. Additionally, TEM8/ANTXR1 contributes to the angiogenic response of cultured umbilical vein endothelial cells, emphasizing its involvement in vascular development. Notably, in microbial infection scenarios, TEM8/ANTXR1 serves as a receptor for the protective antigen (PA) of Bacillus anthracis, highlighting its dual role in cellular processes and host-pathogen interactions.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9H6X2-4 (E33-K321)

  • Gene ID
    84168
  • Molecular Construction
    • N-term
    • TEM8 (E33-K321)
      Accession # Q9H6X2-4
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    ANTXR1; FLJ10601; ANTXR Cell Adhesion Molecule 1; Tumor Endothelial Marker 8 Precursor; Anthrax Toxin Receptor 1; Anthrax Toxin Receptor; TEM8; ANTXR1 Protein; ATR; 2310008J16Rik; Tumor Endothelial Marker 8; 2810405N18Rik; FLJ21776; GAPO

  • AA Sequence

    EDGGPACYGGFDLYFILDKSGSVLHHWNEIYYFVEQLAHKFISPQLRMSFIVFSTRGTTLMKLTEDREQIRQGLEELQKVLPGGDTYMHEGFERASEQIYYENRQGYRTASVIIALTDGELHEDLFFYSEREANRSRDLGAIVYCVGVKDFNETQLARIADSKDHVFPVNDGFQALQGIIHSILKKSCIEILAAEPSTICAGESFQVVVRGNGFRHARNVDRVLCSFKINDSVTLNEKPFSVEDTYLLCPAPILKEVGMKAALQVSMNDGLSFISSSVIITTTHCSLHK

  • Predicted Molecular Mass

    33.6 kDa

  • Molecular Weight

    Approximately 35-45 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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