Latent TGF beta 1/TGFB1 Protein, Cynomolgus (HEK293, His)
Latent TGF beta 1/TGFB1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Latent TGF beta 1/TGFB1 protein, expressed by HEK293, with C-His tag.
- Species: Cynomolgus
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Latent TGF beta 1/TGFB1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Latent TGF beta 1/TGFB1 protein, expressed by HEK293, with C-His tag.
Background
TGF-B1 is known to be secreted in the inactive, latent form. And latent TGF beta 1 (latent TGFB1) is comprised of three distinct components: mature TGFB1 which is a disulphide-bonded dimer, the N-terminal remnant of theTGFB1 precursor and a novel type of protein denoted the latent TGFB1-binding protein (LTBP)[2].
Although latent TGF beta 1 is converted into its biologically active form by various mechanisms, but all mechanisms involve dissociation of TGFB1 from LAP-B1 in the soluble SLC (small latent complex) and/or the ECM bound LLC (large latent complex). In addition, Proteolytic cleavage is the most prominent cellular mechanism of latent TGFB1 activation. Latent TGF beta 1 associates with the extracellular matrix (ECM) via LTBP. LTBPs are components of the ECM, so that the proteolytic cleavage of LTBP can lead to the release of latent TGF-beta 1 from the matrix. Besides, the proteolytic cleavage of LLC and liberation of active TGFB1 is performed by BMP-1, by a variety of matrix metalloproteinases (MMPs)[1][3].
Technical Parameters
-
Species Cynomolgus
-
Source HEK293
-
Tag C-His
-
Accession
XP_005589396.1 (L30-S390)
-
Gene ID102129546
-
Protein Length
Full Length of Mature Protein
-
Synonyms
TGFB1; Transforming Growth Factor Beta1; Prev. TGFB; Camurati-Engelmann Disease; Prev. DPD1; Latency-Associated Peptide; TGFbeta; TGF-Beta-1; CED; TGF-Beta1; Transforming Growth Factor Beta-1 Proprotein; IBDIMDE; Prepro-Transforming Growth Factor Beta-1;
-
AA Sequence
LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSKDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
-
Predicted Molecular Mass
42.7 kDa
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
