Latent TGF beta 1/TGFB1 Protein, Cynomolgus (HEK293, His)

Latent TGF beta 1/TGFB1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Latent TGF beta 1/TGFB1 protein, expressed by HEK293, with C-His tag.

TGF-B1 is known to be secreted in the inactive, latent form. And latent TGF beta 1 (latent TGFB1) is comprised of three distinct components: mature TGFB1 which is a disulphide-bonded dimer, the N-terminal remnant of theTGFB1 precursor and a novel type of protein denoted the latent TGFB1-binding protein (LTBP)^[2].
Although latent TGF beta 1 is converted into its biologically active form by various mechanisms, but all mechanisms involve dissociation of TGFB1 from LAP-B1 in the soluble SLC (small latent complex) and/or the ECM bound LLC (large latent complex). In addition, Proteolytic cleavage is the most prominent cellular mechanism of latent TGFB1 activation. Latent TGF beta 1 associates with the extracellular matrix (ECM) via LTBP. LTBPs are components of the ECM, so that the proteolytic cleavage of LTBP can lead to the release of latent TGF-beta 1 from the matrix. Besides, the proteolytic cleavage of LLC and liberation of active TGFB1 is performed by BMP-1, by a variety of matrix metalloproteinases (MMPs)^[1][3].

Cynomolgus

HEK293

C-His

XP_005589396.1 (L30-S390)

102129546

Full Length of Mature Protein

LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSKDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.