TPBG/5T4 Protein, Mouse (HEK293, Fc)
TPBG/5T4 protein acts as an inhibitor of Wnt/β-catenin signaling, possibly by interacting indirectly with LRP6 and hindering Wnt3a-dependent LRP6 internalization. This suggests that TPBG/5T4 plays a key role in regulating the complex Wnt/β-catenin signaling pathway and influencing cellular responses related to this pathway. TPBG/5T4, Mouse (HEK293, Fc) is the recombinant mouse-derived TPBG/5T4 protein, expressed by HEK293, with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
TPBG/5T4 protein acts as an inhibitor of Wnt/β-catenin signaling, possibly by interacting indirectly with LRP6 and hindering Wnt3a-dependent LRP6 internalization. This suggests that TPBG/5T4 plays a key role in regulating the complex Wnt/β-catenin signaling pathway and influencing cellular responses related to this pathway. TPBG/5T4, Mouse (HEK293, Fc) is the recombinant mouse-derived TPBG/5T4 protein, expressed by HEK293, with C-His labeled tag.
Background
The TPBG/5T4 protein appears to operate as an inhibitor of Wnt/beta-catenin signaling, potentially exerting its inhibitory effects by indirectly interacting with LRP6 and impeding Wnt3a-dependent LRP6 internalization. This suggests that TPBG/5T4 may play a critical role in modulating the intricate Wnt/beta-catenin signaling pathway, influencing cellular responses and functions associated with this pathway. A comprehensive investigation into the specific molecular mechanisms underlying the interaction between TPBG/5T4 and LRP6, as well as its impact on Wnt3a-dependent LRP6 internalization, could provide valuable insights into the functional significance of TPBG/5T4 in the regulation of Wnt/beta-catenin signaling and its potential implications for cellular processes.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-hFc
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Accession
Q9Z0L0 (S32-S361)
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Gene ID21983
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Molecular Construction
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N-term
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TLR8 (S32-S361)
Accession # Q9Z0L0 -
hFc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
TPBG; 5T4 Oncofetal Antigen; Trophoblast Glycoprotein; 5T4-AG; 5T4; WAIF1; 5T4 Oncofetal Trophoblast Glycoprotein; M6P1; Wnt-Activated Inhibitory Factor 1; 5T4AG; 5T4 Oncotrophoblast Glycoprotein
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AA Sequence
SAPSSSVPSSSTSPAAFLASGSAQPPPAERCPAACECSEAARTVKCVNRNLLEVPADLPPYVRNLFLTGNQMTVLPAGAFARQPPLADLEALNLSGNHLKEVCAGAFEHLPGLRRLDLSHNPLTNLSAFAFAGSNASVSAPSPLEELILNHIVPPEDQRQNGSFEGMVAFEGMVAAALRSGLALRGLTRLELASNHFLFLPRDLLAQLPSLRYLDLRNNSLVSLTYASFRNLTHLESLHLEDNALKVLHNSTLAEWHGLAHVKVFLDNNPWVCDCYMADMVAWLKETEVVPDKARLTCAFPEKMRNRGLLDLNSSDLDCDAVLPQSLQTS
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Predicted Molecular Mass
62.2 kDa
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Molecular Weight
Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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