TSLP Protein, Human (R127A, R130A, HEK293, mFc)

TSLP Protein, a cytokine, stimulates T-cell-attracting chemokine release from monocytes and enhances CD11c(+) dendritic cell maturation. It directly activates mast cells, possibly inducing allergic inflammation. TSLP may also possess antimicrobial properties in the oral cavity and on the skin. TSLP Protein, Human (R127A, R130A, HEK293, mFc) is the recombinant human-derived TSLP protein, expressed by HEK293 , with C-mFc labeled tag and R127A, R130A mutation. The total length of TSLP Protein, Human (R127A, R130A, HEK293, mFc) is 131 a.a., with molecular weight of 50-58 kDa.

The TSLP protein is a cytokine that stimulates the release of T-cell-attracting chemokines from monocytes, with a particular effect on enhancing the maturation of CD11c(+) dendritic cells. Additionally, this protein can directly activate mast cells, potentially leading to the induction of allergic inflammation. It is also suggested that TSLP may have antimicrobial properties in the oral cavity and on the skin.

Immobilized Human TSLP R Fc at 5 μg/mL (100 μL/well) can bind Human TSLP (R127A, R130A) mFc with a linear range of 0.3-1.7 ng/mL.

Human

HEK293

C-mFc

Q969D9 (Y29-Q159, R127A, R130A)

85480  [NCBI]

50-58 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.