UBAP1 Protein, Human (His)
The UBAP1 protein is a component of the ESCRT-I complex (PubMed:21757351, PubMed:22405001, PubMed:31203368) and binds to ubiquitinated cargo proteins, promoting their sorting into multivesicular bodies (MVBs) (PubMed:21757351, PubMed :22405001). UBAP1 Protein, Human (His) is the recombinant human-derived UBAP1 protein, expressed by E. coli , with C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The UBAP1 protein is a component of the ESCRT-I complex (PubMed:21757351, PubMed:22405001, PubMed:31203368) and binds to ubiquitinated cargo proteins, promoting their sorting into multivesicular bodies (MVBs) (PubMed:21757351, PubMed :22405001). UBAP1 Protein, Human (His) is the recombinant human-derived UBAP1 protein, expressed by E. coli , with C-6*His labeled tag.
UBAP1 protein functions as a pivotal component of the ESCRT-I complex, a crucial regulator of vesicular trafficking processes. Within this complex, UBAP1 plays a central role in binding to ubiquitinated cargo proteins, facilitating the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). This activity is essential for the proper cellular sorting and degradation of ubiquitinated cell-surface proteins, including EGFR and BST2, through the proteasomal pathway. UBAP1 participates in an ESCRT-I complex, alongside TSG101, VPS28, and VPS37A, forming a well-balanced 1:1:1:1 stoichiometry. Additionally, UBAP1 interacts with PTPN23 and ubiquitinated proteins through its UBA domains, underscoring its significance in coordinating cellular processes related to vesicle trafficking, protein sorting, and degradation.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
Q9NZ09-1 (M1-S502)
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Molecular Construction
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N-term
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UBAP1 (M1-S502)
Accession # Q9NZ09-1 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
UBAP1; Ubiquitin Associated Protein 1; UBAP; Ubiquitin‑Associated Protein 1; Nasopharyngeal Carcinoma‑Associated Gene 20 Protein; UBAP‑1; Ubiquitin Associated Protein; SPG80; NAG20; UAP
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AA Sequence
MASKKLGADFHGTFSYLDDVPFKTGDKFKTPAKVGLPIGFSLPDCLQVVREVQYDFSLEKKTIEWAEEIKKIEEAEREAECKIAEAEAKVNSKSGPEGDSKMSFSKTHSTATMPPPINPILASLQHNSILTPTRVSSSATKQKVLSPPHIKADFNLADFECEEDPFDNLELKTIDEKEELRNILVGTTGPIMAQLLDNNLPRGGSGSVLQDEEVLASLERATLDFKPLHKPNGFITLPQLGNCEKMSLSSKVSLPPIPAVSNIKSLSFPKLDSDDSNQKTAKLASTFHSTSCLRNGTFQNSLKPSTQSSASELNGHHTLGLSALNLDSGTEMPALTSSQMPSLSVLSVCTEESSPPNTGPTVTPPNFSVSQVPNMPSCPQAYSELQMLSPSERQCVETVVNMGYSYECVLRAMKKKGENIEQILDYLFAHGQLCEKGFDPLLVEEALEMHQCSEEKMMEFLQLMSKFKEMGFELKDIKEVLLLHNNDQDNALEDLMARAGAS
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Predicted Molecular Mass
56.2 kDa
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Molecular Weight
Approximately 60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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