glycoprotein E/gE Protein, Varicella zoster virus (HEK293, His)
Based on 1 Customer Validation
Glycoprotein E/gE proteins bind to the host cell entry receptor IDE. glycoprotein E/gE Protein, Varicella zoster virus (HEK293, His) is the recombinant virus-derived glycoprotein E/gE protein, expressed by HEK293, with C-His labeled tag.
- Species: Virus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Glycoprotein E/gE proteins bind to the host cell entry receptor IDE. glycoprotein E/gE Protein, Varicella zoster virus (HEK293, His) is the recombinant virus-derived glycoprotein E/gE protein, expressed by HEK293, with C-His labeled tag.
Background
Envelope glycoprotein E (gE) functions as a key player in the viral life cycle by binding to the potential host cell entry receptor IDE. Particularly crucial in epithelial cells, the gE/gI heterodimer is essential for the efficient cell-to-cell spread of the virus, facilitating the sorting of nascent virions to cell junctions. Once positioned at these junctions, rapid virus dissemination to adjacent cells occurs through interactions with cellular receptors that accumulate at these sites. Additionally, gE/gI is implicated in basolateral spread in polarized cells. In neuronal cells, the gE/gI heterodimer plays a vital role in the anterograde spread of the infection throughout the host nervous system. Teaming up with US9, this heterodimer is involved in the sorting and transport of viral structural components toward axon tips, contributing to the orchestrated progression of the infection.
Verified Bioactivity
Measured by its binding ability in a functional ELISA.Immobilized Varicella zoster virus (strain Oka vaccine) Envelope Glycoprotein E (gE), His Tag at 1 μg/mL (100 μL/well) can bind Anti-Glycoprotein E (VZV) Antibody, Human IgG1 (4G2) with a linear range of ≤0.072 μg/mL.
Technical Parameters
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Species Virus
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Source HEK293
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Tag C-His
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Accession
Q9J3M8-1 (S31-A546)
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Gene ID/
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Molecular Construction
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N-term
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gE (S31-A546)
Accession # Q9J3M8-1 -
His
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C-term
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Protein Length
Partial
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Synonyms
US8; gE; gE-2; gE; gE-2; Envelope glycoprotein E
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AA Sequence
SVLRYDDFHIDEDKLDTNSVYEPYYHSDHAESSWVNRGESSRKAYDHNSPYIWPRNDYDGFLENAHEHHGVYNQGRGIDSGERLMQPTQMSAQEDLGDDTGIHVIPTLNGDDRHKIVNVDQRQYGDVFKGDLNPKPQGQRLIEVSVEENHPFTLRAPIQRIYGVRYTETWSFLPSLTCTGDAAPAIQHICLKHTTCFQDVVVDVDCAENTKEDQLAEISYRFQGKKEADQPWIVVNTSTLFDELELDPPEIEPGVLKVLRTEKQYLGVYIWNMRGSDGTSTYATFLVTWKGDEKTRNPTPAVTPQPRGAEFHMWNYHSHVFSVGDTFSLAMHLQYKIHEAPFDLLLEWLYVPIDPTCQPMRLYSTCLYHPNAPQCLSHMNSGCTFTSPHLAQRVASTVYQNCEHADNYTAYCLGISHMEPSFGLILHDGGTTLKFVDTPESLSGLYVFVVYFNGHVEAVAYTVVSTVDHFVNAIEERGFPPTAGQPPATTKPKEITPVNPGTSPLLRYAAWTGGLA
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Predicted Molecular Mass
60.2 kDa
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Molecular Weight
Approximately 54-69 kDa, based on SDS-PAGE under non reducing (N) conditions, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Monomer
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with 10% trehalose as protectant.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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