VCAM-1 Protein, Cynomolgus (HEK293, His)
VCAM-1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived VCAM-1 protein, expressed by HEK293, with C-His tag.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
VCAM-1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived VCAM-1 protein, expressed by HEK293, with C-His tag.
Background
VCAM-1 is a cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells, playing a crucial role in immune surveillance and inflammation. It acts as a major regulator of leukocyte adhesion to the endothelium through interactions with various integrins. During inflammatory responses, VCAM-1 binds ligands on activated endothelial cells, triggering calcium channel activation and plasma membrane-associated small GTPase RAC1, leading to leukocyte transendothelial migration. Additionally, it serves as a quality-control checkpoint for bone marrow entry by providing a 'don't-eat-me' signal in the context of major histocompatibility complex (MHC) class-I presentation.
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-His
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Accession
G7NV32 (F25-E698)
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Gene ID/
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Molecular Construction
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N-term
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VCAM-1 (F25-E698)
Accession # G7NV32 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
VCAM1; Vascular Cell Adhesion Molecule 1; Vascular Cell Adhesion Protein 1; CD106; CD106 Antigen; INCAM-100; V-CAM 1; VCAM-1
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AA Sequence
FKIETTPESKSLAQIGDSVSLTCSTTGCESPSFSWRTQIDSPLNGKVRNEGTTSTLTMNPVSFGNEHSYLCTATCGSRKLEKGIQVEIYSFPRDPEIHLSGPLEAGKPITVKCLVADVYPFDRLEIDLMKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTEREAVEELQVYISPKNTVISVNPSTRLQEGGSVTMTCSSEGLPPPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVVLTCTVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGRKKLEKGIQVELYSFPRDPEIEMRGVLVSESPITVSCKVSSVYPLDRLEIELLKGETVLENKEFLEDTDMKSLENKSLEATFIPTTEDTGKALVCQAKLHMDEMEFEPKQRQSTQTLYVNVAPRDTTILVSPSSILEEGSSVNMTCLSHGLPAPKILWSRQLPNGELQSLSEDATLNLISTKMEDSGVYVCEGINQAGRSRKEVELIIQATPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAQTGDTVLKSIDGAYTIQKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPE
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Predicted Molecular Mass
76.1 kDa
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Molecular Weight
Approximately 85-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Monomer
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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