VCAM-1 Protein, Cynomolgus (HEK293, His)

VCAM-1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived VCAM-1 protein, expressed by HEK293, with C-His tag.

VCAM-1 is a cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells, playing a crucial role in immune surveillance and inflammation. It acts as a major regulator of leukocyte adhesion to the endothelium through interactions with various integrins. During inflammatory responses, VCAM-1 binds ligands on activated endothelial cells, triggering calcium channel activation and plasma membrane-associated small GTPase RAC1, leading to leukocyte transendothelial migration. Additionally, it serves as a quality-control checkpoint for bone marrow entry by providing a 'don't-eat-me' signal in the context of major histocompatibility complex (MHC) class-I presentation.

Cynomolgus

HEK293

C-His

G7NV32 (F25-E698)

/

Partial

FKIETTPESKSLAQIGDSVSLTCSTTGCESPSFSWRTQIDSPLNGKVRNEGTTSTLTMNPVSFGNEHSYLCTATCGSRKLEKGIQVEIYSFPRDPEIHLSGPLEAGKPITVKCLVADVYPFDRLEIDLMKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTEREAVEELQVYISPKNTVISVNPSTRLQEGGSVTMTCSSEGLPPPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVVLTCTVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGRKKLEKGIQVELYSFPRDPEIEMRGVLVSESPITVSCKVSSVYPLDRLEIELLKGETVLENKEFLEDTDMKSLENKSLEATFIPTTEDTGKALVCQAKLHMDEMEFEPKQRQSTQTLYVNVAPRDTTILVSPSSILEEGSSVNMTCLSHGLPAPKILWSRQLPNGELQSLSEDATLNLISTKMEDSGVYVCEGINQAGRSRKEVELIIQATPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAQTGDTVLKSIDGAYTIQKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPE

Approximately 85-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.