VRK1 Protein, Human (Active, sf9, His-GST)
The VRK1 protein is a serine/threonine kinase that complexly regulates the cell cycle, nuclear condensation, and transcription. It critically induces Golgi disassembly during the cell cycle via PLK3 phosphorylation, thereby affecting Golgi fragmentation. VRK1, Human (Active, sf9, His-GST) is the recombinant human-derived VRK1 protein, expressed by Sf9 insect cells, with N-6*His;N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
The VRK1 protein is a serine/threonine kinase that complexly regulates the cell cycle, nuclear condensation, and transcription. It critically induces Golgi disassembly during the cell cycle via PLK3 phosphorylation, thereby affecting Golgi fragmentation. VRK1, Human (Active, sf9, His-GST) is the recombinant human-derived VRK1 protein, expressed by Sf9 insect cells, with N-6*His;N-GST labeled tag.
Background
VRK1, a serine/threonine kinase, intricately participates in cell cycle regulation, nuclear condensation, and transcriptional control. Through its diverse functions, VRK1 exhibits a multifaceted impact on cellular processes. Notably, it plays a crucial role in Golgi disassembly during the cell cycle, a process initiated by phosphorylation from PLK3 during mitosis, ultimately leading to Golgi fragmentation. VRK1 further modulates the intricate network of p53/TP53, phosphorylating 'Thr-18' and potentially impeding the interaction between p53/TP53 and MDM2. In response to DNA damage, VRK1 phosphorylates KAT5, fostering its association with chromatin and enhancing histone acetyltransferase activity. Moreover, VRK1 influences nuclear dynamics by phosphorylating BANF1, disrupting its DNA-binding capability, reducing its interaction with LEM domain-containing proteins, and triggering its relocalization from the nucleus to the cytoplasm. Additionally, VRK1 targets ATF2, activating its transcriptional activity and further expanding the regulatory repertoire of this versatile kinase.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-6*His;N-GST
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Accession
Q99986 (M1-K396)
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Gene ID7443
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Molecular Construction
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N-term
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6*His-GST
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VRK1 (M1-K396)
Accession # Q99986 -
C-term
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Protein Length
Full Length
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Synonyms
VRK1; VRK Serine/Threonine Kinase 1; Serine/Threonine-Protein Kinase VRK1; Vaccinia Related Kinase 1; Vaccinia-Related Kinase 1; Non-Specific Serine/Threonine Protein Kinase; Vaccinia Virus B1R-Related Kinase 1; HMNR10; PCH1A; PCH1
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Predicted Molecular Mass
75.6 kDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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