Zaire ebolavirus VP30 Protein (His)
Zaire ebolavirus VP30 Protein (His) is the recombinant virus-derived Zaire ebolavirus VP30 protein, expressed by E. coli, with N-6*His tag.
- Species: Virus
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Zaire ebolavirus VP30 Protein (His) is the recombinant virus-derived Zaire ebolavirus VP30 protein, expressed by E. coli, with N-6*His tag.
Background
Zaire ebolavirus VP30 is a multifunctional protein that acts as a viral transcriptional activator. It promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA, clamping the RNA template and the VP35-polymerase L complex together, thereby increasing the polymerase affinity for the RNA template to boost transcription initiation despite RNA secondary structures. Additionally, VP30 assists stop-start transcription at gene junctions to promote downstream gene transcription (by similar). Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop (by similar). Interaction with host RBBP6 disrupts NP-VP30 binding and inhibits viral RNA synthesis. VP30 also suppresses RNA silencing by interacting with host DICER1 and TARBP2/TRBP.
Technical Parameters
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Species Virus
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Source E. coli
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Tag N-6*His
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Accession
Q77DJ5 (M1-P288)
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Gene ID911826
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Molecular Construction
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N-term
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6*His
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VP30 (M1-P288)
Accession # Q77DJ5 -
C-term
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Protein Length
Full Length
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Synonyms
VP30; Transcriptional activator VP30; EbolaVP30; eVP30; Minor nucleoprotein VP30
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AA Sequence
MEASYERGRPRAARQHSRDGHDHHVRARSSSRENYRGEYRQSRSASQVRVPTVFHKKRVEPLTVPPAPKDICPTLKKGFLCDSSFCKKDHQLESLTDRELLLLIARKTCGSVEQQLNITAPKDSRLANPTADDFQQEEGPKITLLTLIKTAEHWARQDIRTIEDSKLRALLTLCAVMTRKFSKSQLSLLCETHLRREGLGQDQAEPVLEVYQRLHSDKGGSFEAALWQQWDRQSLIMFITAFLNIALQLPCESSAVVVSGLRTLVPQSDNEEASTNPGTCSWSDEGTP
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Predicted Molecular Mass
38 kDa
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Structure/Form
Monomer
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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