1. Recombinant Proteins
  2. Viral Proteins
  3. Zika Virus Proteins
  4. Zika Virus Non-structural Protein 1
  5. Zika virus NS1 Protein (HEK293, His)

Zika virus NS1 Protein (HEK293, His)

Cat. No.: HY-P74455
COA Handling Instructions

Genome polyprotein is a smaller protein chain of covalent linkages that is a key component of virus survival and replication. Zika virus NS1 Protein (HEK293, His) is the recombinant Virus-derived Zika virus NS1 protein, expressed by HEK293 , with N-His labeled tag. The total length of Zika virus NS1 Protein (HEK293, His) is 362 a.a., with molecular weight of ~43.5 kDa.

For research use only. We do not sell to patients.

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Description

Genome polyprotein is a smaller protein chain of covalent linkages that is a key component of virus survival and replication. Zika virus NS1 Protein (HEK293, His) is the recombinant Virus-derived Zika virus NS1 protein, expressed by HEK293 , with N-His labeled tag. The total length of Zika virus NS1 Protein (HEK293, His) is 362 a.a., with molecular weight of ~43.5 kDa.

Background

Genome polyprotein is a series of protein units with similar or different functions that have been widely utilized by single-celled or multi-cellular organisms as concentrators of countless molecular activities. Genome polyprotein is a small protein chain that is covalently linked, and it is a common means of organizing the protein set of viruses (including HIV) in nature. As the signal peptide of NS4B, genome polyprotein is essential for the anti-interferon activity of NS4B. Genome polyprotein inhibits RNA silencing by interfering with host Dicer. Genome polyprotein may play a role in viral budding. Genome polyprotein exerts cytotoxic effects by activating the mitochondrial apoptosis pathway through the M ectodomain. Genome polyprotein may display viral protein activity[1][2].

Species

Virus

Source

HEK293

Tag

N-His

Accession

ALU33341 (V796-L1157)

Gene ID

/

Molecular Construction
N-term
His
ZIKV NS1 (V796-L1157)
Accession # ALU33341
C-term
Synonyms
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 protein
AA Sequence

VGCSVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGICGISSVSRMENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVPVNELPHGWKAWGKSHFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDHGFGVFHTSVWLKVREDYSLECDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWRLKRAHLIEMKTCEWPKSHTLWTDGIEESDLIIPKSLAGPLSHHNTREGYRTQMKGPWHSEELEIRFEECPGTKVHVEETCGTRGPSLRSTTASGRVIEEWCCRECTMPPLSFRAKDGCWYGMEIRPRKEPESNLVRSMVTAGSTDHMDHFSL

Molecular Weight

Approximately 43.5 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Zika virus NS1 Protein (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Zika virus NS1 Protein (HEK293, His)
Cat. No.:
HY-P74455
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