Ribavirin 5'-triphosphate ammonium
Ribavirin 5'-triphosphate ammonium is an inhibitor of the dengue virus NS5 2'-O-methyltransferase NS5 domain (NS5MTaseDV) (IC50 = 101 μM; Kd = 55 μM). Ribavirin 5'-triphosphate ammonium blocks RNA cap methylation by competitively binding to the GTP-binding site of NS5MTaseDV, thereby inhibiting the viral mRNA 2'-O-methyltransferase activity. Ribavirin 5'-triphosphate ammonium can be used for research on dengue fever.
For research use only. We do not sell to patients.
- CAS No.: 332153-24-9
- Formula: C8H27N8O14P3
- Molecular Weight:552.27
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
NS5MTaseDV 101 μM (IC50) |
NS5MTaseDV 55 μM (Kd) |
In Vitro
Ribavirin 5′-triphosphate (50-750 μM; 30 min to 3 h) ammonium inhibits dengue virus NS5MTaseDV RNA 2′-O-methyltransferase activity with an IC50 of 101 μM[1].
Ribavirin 5′-triphosphate (100-1000 μM) ammonium competes with GTP for binding to dengue virus NS5MTaseDV, with an apparent Kd of 55 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 332153-24-9
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Molecular Weight 552.27
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Formula C8H27N8O14P3
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SMILES
O[C@@H]1[C@H](O)[C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O[C@H]1N2C=NC(C(N)=O)=N2.N.N.N.N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ribavirin 5'-triphosphate ammonium Related Classifications
HY-134016B Related Classifications
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