ROX alkyne, 6-isomer
ROX alkyne, 6-isomer is an alkyne derivative of the bright red-emitting dye ROX (Rhodamine X, Rhodamine 101). Due to its quantum yield approaching unity, ROX is a popular dye for qPCR and microscopy applications. It is used for fluorescent labeling of azide-containing biomolecules via copper-catalyzed click reaction.
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- CAS No.: 2097422-22-3
- Formula: C36H33N3O4
- Molecular Weight:571.66
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 2097422-22-3
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Molecular Weight 571.66
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Formula C36H33N3O4
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SMILES
O=C(C1=C(C2=C(C=C3C4=C5CCCN4CCC3)C5=[O+]C6=C2C=C7C8=C6CCCN8CCC7)C=C(C(NCC#C)=O)C=C1)[O-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ROX alkyne, 6-isomer Related Classifications
HY-D2743 Related Classifications
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