Salvianolic acid D

Name: Salvianolic acid D
Brand: MedChemExpress
SKU: HY-N0320
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0320 Purity: 95.15%: Data Sheet
COA

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Salvianolic acid D is a depside. Salvianolic acid D can be isolated from Salvia miltiorrhiza (Danshen). Salvianolic acid D promotes the expression of Bcl-2, and inhibits the expressions of Bax, Cleaved caspase-3 and -9. Salvianolic acid D reduces the expression levels of TLR4, MyD88 and TRAF6 proteins both in vitro and in vivo, and significantly inhibits the nuclear translocation of NF-κB. Salvianolic acid D inhibits the cytoplasmic translocation of HMGB1. Salvianolic acid D suppresses inflammatory responses and alleviates cerebral ischemia-reperfusion injury. Salvianolic acid D serves as a potential antiplatelet active component.

For research use only. We do not sell to patients.

Salvianolic acid D Chemical Structure

CAS No. : 142998-47-8

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
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50 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Salvianolic acid D:

Salvianolic acid D (Standard) Get quote

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•Related Screening Libraries:

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•Related Proteins:

View All Bcl-2 Family Isoform Specific Products:

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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View All Isoforms

TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

View All NF-κB Isoform Specific Products:

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Bcl-2

Bax

Caspase-9

Caspase-3

TLR4

In Vitro

Salvianolic acid D (0.2-5 μM; 24 h) increases OGD/R-injured PC12 cell viability, inhibits apoptosis, reduces inflammatory cytokine production, and blocks HMGB1/TLR4/MyD88/NF-κB signaling when applied at 0.2, 1, or 5 μM for 24 hours post-OGD/R^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:	PC12
Concentration:	0.2-5 μM
Incubation Time:	24 h
Result:	Inhibited the production of TNF-α, IL-1β, and IL-6 induced by OGD/R injury.

Apoptosis Analysis^[1]

Cell Line:	PC12
Concentration:	0.2-5 μM
Incubation Time:	24 h
Result:	Concentration-dependently decreased the levels of cleaved caspase-3. Restrained cleaved caspase-9 induction process effectively. Downregulated the release of cytochrome c.

In Vivo

Salvianolic acid D (1-15 mg/kg; intravenous injection; twice) dose-dependently alleviates cerebral ischemia-reperfusion injury in male Sprague-Dawley rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague-Dawley (SD) (male, 250 g, cerebral ischemia-reperfusion injury via MCAO/R model)^[1]
Dosage:	1 mg/kg; 3 mg/kg; 15 mg/kg
Administration:	i.v.; twice (at onset of reperfusion and 12 h postreperfusion)
Result:	Significantly reduced neurological deficit scores compared to I/R rats (P < 0.05 for 15 mg/kg, P < 0.01 for 1 mg/kg and 3 mg/kg). Significantly reduced infarct volume at 15 mg/kg and 3 mg/kg doses (P < 0.01 for both). Dose-dependently reduced brain water content compared to I/R rats (P < 0.05 for 1 mg/kg, P < 0.01 for 3 mg/kg and 15 mg/kg). Significantly reduced Evans blue extravasation (P < 0.01 for all doses), indicating reduced blood-brain barrier permeability. Significantly reduced TUNEL-positive cell percentage at 3 mg/kg and 15 mg/kg doses (P < 0.01 for both). Reduced Bax/Bcl-2 ratio at all doses (P < 0.01 for all). Reduced cleaved caspase-3 intensity at 1 mg/kg (P < 0.05), while 3 mg/kg and 15 mg/kg reduced cleaved caspase-3 and cleaved caspase-9 intensity (P < 0.01 for all). Reduced cytochrome c intensity at 3 mg/kg (P < 0.05) and 15 mg/kg (P < 0.01). Reduced brain tissue levels of TNF-α, IL-1β, and IL-6 at all doses (P < 0.01 for most comparisons). Dose-dependently reduced serum HMGB1 levels at all doses (P < 0.01 for all). Reduced TLR4, MyD88, and TRAF6 protein intensity in cortex and hippocampus at all doses (P < 0.05 or P < 0.01 vs. I/R rats). Reduced nuclear p-NF-κB p65/NF-κB p65 ratio in cortex and hippocampus (P < 0.01 for 3 mg/kg and 15 mg/kg, P < 0.05 for 1 mg/kg in some regions). Reduced cytoplasmic HMGB1 intensity and increased nuclear HMGB1 intensity in cortex and hippocampus at all doses (P < 0.05 or P < 0.01 vs. I/R rats). Inhibited HMGB1 cytoplasmic translocation in neurons, astrocytes, and microglia in cortex and hippocampus at 15 mg/kg dose.

Molecular Weight

418.35

Formula

C₂₀H₁₈O₁₀

CAS No.

142998-47-8

Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=CC=C(/C=C/C(O[C@@H](C(O)=O)CC2=CC(O)=C(O)C=C2)=O)C(CC(O)=O)=C1O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (119.52 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3903 mL	11.9517 mL	23.9034 mL
5 mM	0.4781 mL	2.3903 mL	4.7807 mL
10 mM	0.2390 mL	1.1952 mL	2.3903 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 95.15%

Select Batch:

Data Sheet (280 KB) SDS (254 KB)

COA (249 KB) HNMR (93 KB) CNMR (94 KB) RP-HPLC (225 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang W, et al. Salvianolic Acid D Alleviates Cerebral Ischemia-Reperfusion Injury by Suppressing the Cytoplasmic Translocation and Release of HMGB1-Triggered NF-κB Activation to Inhibit Inflammatory Response. Mediators Inflamm. 2020;2020:9049614. Published 2020 Jan 22.

[2]. Ai CB, et al. Salvianolic Acids D and E: Two New Depsides from Salvia miltiorrhiza. Planta Med. 1992;58(2):197-199. [Content Brief]

[3]. Chen Y, et al. A Platelet/CMC coupled with offline UPLC-QTOF-MS/MS for screening antiplatelet activity components from aqueous extract of Danshen. J Pharm Biomed Anal. 2016;117:178-183. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.3903 mL	11.9517 mL	23.9034 mL	59.7586 mL
	5 mM	0.4781 mL	2.3903 mL	4.7807 mL	11.9517 mL
	10 mM	0.2390 mL	1.1952 mL	2.3903 mL	5.9759 mL
	15 mM	0.1594 mL	0.7968 mL	1.5936 mL	3.9839 mL
	20 mM	0.1195 mL	0.5976 mL	1.1952 mL	2.9879 mL
	25 mM	0.0956 mL	0.4781 mL	0.9561 mL	2.3903 mL
	30 mM	0.0797 mL	0.3984 mL	0.7968 mL	1.9920 mL
	40 mM	0.0598 mL	0.2988 mL	0.5976 mL	1.4940 mL
	50 mM	0.0478 mL	0.2390 mL	0.4781 mL	1.1952 mL
	60 mM	0.0398 mL	0.1992 mL	0.3984 mL	0.9960 mL
	80 mM	0.0299 mL	0.1494 mL	0.2988 mL	0.7470 mL
	100 mM	0.0239 mL	0.1195 mL	0.2390 mL	0.5976 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.