A Platelet/CMC coupled with offline UPLC-QTOF-MS/MS for screening antiplatelet activity components from aqueous extract of Danshen

  • J Pharm Biomed Anal. 2016 Jan 5;117:178-83. doi: 10.1016/j.jpba.2015.06.009.
Ying Chen  1 ,  Na Zhang  2 ,  Jian Ma  1 ,  Yan Zhu  1 ,  Meng Wang  1 ,  Xiaoming Wang  1 ,  Peng Zhang  3
Affiliations
  • 1. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, PR China.
  • 2. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, PR China; Tianjin International Joint Academy of Biomedicine, Tianjin 300457, PR China.
  • 3. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, PR China. Electronic address: [email protected].
PMID: 26355772 DOI: 10.1016/j.jpba.2015.06.009
Abstract

Platelets play crucial roles in thromboembolic and Cardiovascular Disease. The main platelets membrane receptors include adenosine diphosphate receptors, Thrombin receptors, thromboxane prostanoid receptors and Collagen receptors. In this study, a Platelet/CMC coupled with offline UPLC-QTOF-MS/MS system was built to screen antiplatelet activity components from aqueous extract of Danshen, which serve as an agent of antiplatelet aggregation in Traditional Chinese Medicine. Rosmarinic acid, lithospermic acid, salvianolic acid B, two isomers of salvianolic acid B, salvianolic acid C, salvianolic acid D and salvianolic acid H/I were identified as the potential antiplatelet activity components. Moreover, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid C and danshensu were tested in platelet aggregation in vitro assay. The results suggested their retention time was closely related to the antiplatelet aggregation activities. This study provides a rapid, effective and novel method for screening the potential antiplatelet activity components from Chinese herb medicines.

Keywords
Cell membrane chromatography; Danshen; Platelet; UPLC-QTOF-MS/MS.
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