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Cat. No.: HY-D3389

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sCy5DL-amide

sCy5DL-amide is an amidated fluorescent D-amino acid conjugated to Sulfo-Cyanine 5, and a peptidoglycan labeling agent. sCy5DL-amide incorporates into bacterial peptidoglycan cell wall via transpeptidase-mediated reactions, replacing the fourth or fifth D-amino acid of the peptidoglycan stem peptide for visualization of nascent peptidoglycan biosynthesis. sCy5DL-amide produces clear cell outline, septum labeling, and high localization density in Bacillus subtilis, including a 'V-shape' pattern at cell-cell contact areas. sCy5DL-amide exhibits robust incorporation into Gram-positive bacteria and mycobacteria, with reduced incorporation into Gram-negative bacteria (Ex/Em = 646/666 nm).

For research use only. We do not sell to patients.

Formula: C₃₈H₅₁N₅O₈S₂
Molecular Weight:769.97

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

sCy5DL-amide Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
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Bioz

Biological Activity

Description

In Vitro

sCy5DL-amide (0.5 mM; 20 min) efficiently incorporates into the cell wall of Bacillus subtilis, enabling clear visualization of cell outlines and septa via widefield fluorescence imaging^[1].
sCy5DL-amide (1 mM; 1-2 cell cycle duration) efficiently incorporates into the cell walls of Mycobacterium marinum and Mycobacterium tuberculosis, enabling high-resolution visualization of cell wall ultrastructure via SMLM imaging^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 769.97
Formula C₃₈H₅₁N₅O₈S₂
SMILES

CC1(C)C2=C(C=CC(S(=O)([O-])=O)=C2)N(CCCCCC(NCCCC[C@H](C(N)=O)[NH3+])=O)/C1=C/C=C/C=C/C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhang C, et al. Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids. Methods Mol Biol. 2024;2727:83-94. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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