sCy5DL-amide 

sCy5DL-amide is an amidated fluorescent D-amino acid conjugated to Sulfo-Cyanine 5, and a peptidoglycan labeling agent. sCy5DL-amide incorporates into bacterial peptidoglycan cell wall via transpeptidase-mediated reactions, replacing the fourth or fifth D-amino acid of the peptidoglycan stem peptide for visualization of nascent peptidoglycan biosynthesis. sCy5DL-amide produces clear cell outline, septum labeling, and high localization density in Bacillus subtilis, including a 'V-shape' pattern at cell-cell contact areas. sCy5DL-amide exhibits robust incorporation into Gram-positive bacteria and mycobacteria, with reduced incorporation into Gram-negative bacteria (Ex/Em = 646/666 nm)^[1].

sCy5DL-amide (0.5 mM; 20 min) efficiently incorporates into the cell wall of Bacillus subtilis, enabling clear visualization of cell outlines and septa via widefield fluorescence imaging^[1].
sCy5DL-amide (1 mM; 1-2 cell cycle duration) efficiently incorporates into the cell walls of Mycobacterium marinum and Mycobacterium tuberculosis, enabling high-resolution visualization of cell wall ultrastructure via SMLM imaging^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

769.97

C₃₈H₅₁N₅O₈S₂

CC1(C)C2=C(C=CC(S(=O)([O-])=O)=C2)N(CCCCCC(NCCCC[C@H](C(N)=O)[NH3+])=O)/C1=C/C=C/C=C/C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zhang C, et al. Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids. Methods Mol Biol. 2024;2727:83-94.  [Content Brief]