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Fluo3

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By Targets:	Environmental Pollutants (1) Fluorescent Dye (9) LPL Receptor (1)
By Research Areas:	Inflammation/Immunology (1)
Oligonucleotides:	Emulsifiers (1) Solubilizing Agents (1)

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Oligonucleotides

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-D1005

Poloxamer 407 (F127) 5+ Cited Publications PEG-PPG-PEG, 12600 (Average Mn)	Environmental Pollutants LPL Receptor	Inflammation/Immunology
Poloxamer 407 (F127) is a nonionic surfactant that is 100% active and relatively non-toxic to cells at low concentrations, and frequently used with dye AM esters such as Indo-1 AM, Fura-2 AM, Calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8 AM and Quest Rhod-4 AM, etc. to improve their water solubility. Poloxamer 407 is also a lipoprotein lipase inhibitor .

HY-D0716

Fluo-3AM 10+ Cited Publications Fluo-3-pentaacetoxymethyl ester	Fluorescent Dye	Others
Fluo-3 AM is a fluorecent Ca ²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence .

HY-126821

*Fluo-3*	Fluorescent Dye	Others
Fluo-3 is a cell-impermeable calcium ion (Ca ²⁺) indicator. Fluo-3 itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm) .

HY-129547

Fluo-3 pentapotassium	Fluorescent Dye	Others
Fluo-3 pentapotassium is a cell-impermeable calcium ion (Ca ²⁺) indicator. Fluo-3 pentapotassium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm) .

HY-126821A

Fluo-3 pentaammonium	Fluorescent Dye	Others
Fluo-3 pentaammonium is a cell-impermeable calcium ion (Ca ²⁺) indicator. Fluo-3 pentaammonium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm) .

HY-W127795

Fluo-3 ammonium	Fluorescent Dye	Others
Fluo-3 ammonium is a cell-impermeable calcium ion (Ca ²⁺) indicator. Fluo-3 ammonium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm) .

HY-126821B

Fluo-3 sodium	Fluorescent Dye	Others
Fluo-3 sodium is a cell-impermeable calcium ion (Ca ²⁺) indicator. Fluo-3 sodium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm) .

HY-D1447

Fluo-3FF pentapotassium	Fluorescent Dye	Others
Fluo-3FF pentapotassium is a cell-impermeant fluorescent calcium indicator. Fluo-3FF is a di-fluorinated analog of Fluo-3 with a 100-fold lower affinity than Fluo-3 for calcium (Kds=42 and 0.4 μM, respectively). For its low affinity, Fluo-3FF is used for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations.

HY-DY1095

Fluo-3AM (solution) Fluo-3-pentaacetoxymethyl ester (solution)	Fluorescent Dye	Others
Fluo-3AM (solution) is a fluorecent Ca ²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence . Solvent and concentration: DMSO: 5 mM

HY-D1755

Fluo-3FF AM	Fluorescent Dye	Others
Fluo-3FF AM is a low affinity (Kd = 42 μM) fluorescent Ca ²⁺ sensitive indicator (Abs/Em = 462 nm/526 nm). Fluo-3FF AM is Mg ²⁺ insensitive and relatively photostable. Fluo-3FF AM is an analog of Fluo-3FF AM. Fluo-3FF AM is essentially non-fluorescent, but exhibits a strong fluorescence enhancement upon entry into cells and binding to calcium.

Cat. No.	Product Name	Type

HY-D0716

Fluo-3AM 10+ Cited Publications Fluo-3-pentaacetoxymethyl ester	Fluorescent Dye
Fluo-3 AM is a fluorecent Ca ²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence .

HY-126821

*Fluo-3*	Fluorescent Dye
Fluo-3 is a cell-impermeable calcium ion (Ca ²⁺) indicator. Fluo-3 itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm) .

HY-129547

Fluo-3 pentapotassium	Fluorescent Dye
Fluo-3 pentapotassium is a cell-impermeable calcium ion (Ca ²⁺) indicator. Fluo-3 pentapotassium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm) .

HY-126821A

Fluo-3 pentaammonium	Fluorescent Dye
Fluo-3 pentaammonium is a cell-impermeable calcium ion (Ca ²⁺) indicator. Fluo-3 pentaammonium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm) .

HY-D1447

Fluo-3FF pentapotassium	Fluorescent Dye
Fluo-3FF pentapotassium is a cell-impermeant fluorescent calcium indicator. Fluo-3FF is a di-fluorinated analog of Fluo-3 with a 100-fold lower affinity than Fluo-3 for calcium (Kds=42 and 0.4 μM, respectively). For its low affinity, Fluo-3FF is used for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations.

Fluo-3FF pentapotassium

Fluorescent Dye

Fluo-3FF pentapotassium is a cell-impermeant fluorescent calcium indicator. Fluo-3FF is a di-fluorinated analog of Fluo-3 with a 100-fold lower affinity than Fluo-3 for calcium (Kds=42 and 0.4 μM, respectively). For its low affinity, Fluo-3FF is used for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations.

HY-DY1095

Fluo-3AM (solution) Fluo-3-pentaacetoxymethyl ester (solution)	Fluorescent Dye
Fluo-3AM (solution) is a fluorecent Ca ²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence . Solvent and concentration: DMSO: 5 mM

Fluo-3AM (solution)

Fluo-3-pentaacetoxymethyl ester (solution)

Fluorescent Dye

Fluo-3AM (solution) is a fluorecent Ca ²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence .
Solvent and concentration: DMSO: 5 mM

HY-D1755

Fluo-3FF AM	Fluorescent Dye
Fluo-3FF AM is a low affinity (Kd = 42 μM) fluorescent Ca ²⁺ sensitive indicator (Abs/Em = 462 nm/526 nm). Fluo-3FF AM is Mg ²⁺ insensitive and relatively photostable. Fluo-3FF AM is an analog of Fluo-3FF AM. Fluo-3FF AM is essentially non-fluorescent, but exhibits a strong fluorescence enhancement upon entry into cells and binding to calcium.

Cat. No.	Product Name	Type

HY-D1005

Poloxamer 407 (F127) 5+ Cited Publications PEG-PPG-PEG, 12600 (Average Mn)	Biochemical Assay Reagents
Poloxamer 407 (F127) is a nonionic surfactant that is 100% active and relatively non-toxic to cells at low concentrations, and frequently used with dye AM esters such as Indo-1 AM, Fura-2 AM, Calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8 AM and Quest Rhod-4 AM, etc. to improve their water solubility. Poloxamer 407 is also a lipoprotein lipase inhibitor .

HY-W127795

Fluo-3 ammonium	Biochemical Assay Reagents
Fluo-3 ammonium is a cell-impermeable calcium ion (Ca ²⁺) indicator. Fluo-3 ammonium itself is not fluorescent, but it becomes fluorescent after binding to calcium ions (Ex/Em = 488/525 nm) .

Cat. No.	Product Name		Classification

HY-D1005

Poloxamer 407 (F127) 5+ Cited Publications PEG-PPG-PEG, 12600 (Average Mn)		Emulsifiers Solubilizing Agents
Poloxamer 407 (F127) is a nonionic surfactant that is 100% active and relatively non-toxic to cells at low concentrations, and frequently used with dye AM esters such as Indo-1 AM, Fura-2 AM, Calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8 AM and Quest Rhod-4 AM, etc. to improve their water solubility. Poloxamer 407 is also a lipoprotein lipase inhibitor .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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