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protein biotinylation

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Bacterial (1) Biochemical Assay Reagents (6) Casein Kinase (1) Exosomes (1) Fluorescent Dye (2) PROTACs (2) YTHDF (1)
By Research Areas:	Cancer (2) Infection (2)

Inhibitors & Agonists

Fluorescent Dyes

MCE Kits

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-111496

Sulfo-NHS-SS-Biotin sodium 4 Publications Verification	Biochemical Assay Reagents	Others
Sulfo-NHS-SS-biotin sodium is a long-chain cleavable and cell-impermeant amine-reactive *biotinylation* reagent. Sulfo-NHS-SS-biotin sodium can be used for the labeling and purifying of cell-surface protein .

HY-W332335

Sulfosuccinimidyl-6-(biotinamido)hexanoate sodium	Biochemical Assay Reagents	Others
Sulfosuccinimidyl-6-(biotinamido)hexanoate sodium is a protein crosslinker, and is a biotinylation reagentcan for labeling cell surface proteins .

HY-W040254

Biotin-LC-LC-NHS	Fluorescent Dye	Others
Biotin-LC-LC-NHS is a biotinylation reagent that contains an NHS moiety, which can react efficiently with primary amino (-NH2). Biotin-LC-LC-NHS enables simple and efficient biotinylation of antibodies, proteins, and any other primary amine-containing biomolecules in solution. Biotin-LC-LC-NHS can be used to biotinylate small molecules such as Paclitaxel (HY-B0015) for use in binding site characterization.

HY-D1635

Sulfo-NHS-LC-LC-Biotin Biotin-XX-SSE	Fluorescent Dye Exosomes	Infection
Sulfo-NHS-LC-LC-Biotin (Biotin-XX-SSE), a biotin reagent, is used to label the *proteins* exposed to the external leaflet of intact exosomes and contains a larger spacer arm between the biotin and amine reactive linker. The size of this linker helps to overcome steric hindrance and increases labeling efficiency at the crowded exosome surface .

HY-137341

SK-3-91	PROTACs YTHDF	Cancer
SK-3-91 is a PROTAC-type multi-kinase degrader that can jointly induce the degradation of the largest number of unique kinases (more than 125 unique kinases). SK-3-91 induces protein degradation through the ubiquitin biotinylation (E-STUB) pathway. SK-3-91 degrades YTHDF2. SK-3-91 inhibits cell proliferation and induces morphological changes. (Pink: TAE648 ligand (HY-169396); Blue: E3 ligase ligand (HY-131717); Black: Linker (HY-140819). The E3 ligase ligand and linker can form a conjugate (HY-169397)) .

HY-116872

MAC13772 1 Publications Verification	Bacterial	Infection
MAC13772 is a BioA inhibitor with an IC₅₀ of 0.28 μM against E. coli and an IC₅₀ of 0.269 μM against A. baumannii. MAC13772 inhibits bacterial growth by targeting the biotin biosynthesis pathway. MAC13772 can be used in studies related to bacterial infections .

HY-137340

WH-10417-099	PROTACs Casein Kinase	Cancer
WH-10417-099 is a CRBN-based PROTAC multi-kinase degrader that induces the degradation of the maximum number of unique kinases (over 125 unique kinases, such as CSNK1E and PI3Kγ) simultaneously. WH-10417-099 induces protein degradation via the ubiquitin biotinylation (E-STUB) pathway, exhibits synergistic effects with SB-405483 (HY-W1135319). The E3 ligase ligand and linker of WH-10417-099 can form the conjugate Pomalidomide 4'-PEG5-acid (HY-131647) .

HY-148958

SNAP-Biotin	Biochemical Assay Reagents	Others
SNAP-Biotin is a biotin-labeled SNAP tag probe. SNAP-Biotin can be used for protein biotinylation .

HY-W250735

NHS-PEG4-biotinidase resistant biotin	Biochemical Assay Reagents	Others
NHS-PEG4-biotinidase resistant biotin is a biotinylation reagent with a terminal NHS ester. The hydrophilic PEG spacer increases solubility in aqueous media. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

HY-W800679

Mal-Cyclohexyl-PEG3-Biotin	Biochemical Assay Reagents	Others
Mal-Cyclohexyl-PEG3-Biotin is a PEG linker containing a maleimide moiety and a biotin group. Maleimides react specifically with sulfhydryl groups to form a stable thioether linkage when the pH is between 6.5 and 7.5. The biotinylation can react with amine molecules in the presence of activator EDC or HATU. PEG attached to the biotin gives an extended spacer arm that permits the biotin to reach into the binding pocket of the protein. The PEG moiety also increases solubility of Biotin-PEG conjugates considerably.

HY-W590564

Bis-PEG3-biotin	Biochemical Assay Reagents	Others
Bis-PEG3-biotin is a biotinylation reagent with two terminal biotin groups. PEG attached to the biotin gives an extended spacer arm that permits the biotin to reach into the binding pocket of the protein or macromolecule.

Cat. No.	Product Name	Type

HY-111496

Sulfo-NHS-SS-Biotin sodium 4 Publications Verification	Fluorescent Dyes
Sulfo-NHS-SS-biotin sodium is a long-chain cleavable and cell-impermeant amine-reactive *biotinylation* reagent. Sulfo-NHS-SS-biotin sodium can be used for the labeling and purifying of cell-surface protein .

HY-148958

SNAP-Biotin	Fluorescent Dyes
SNAP-Biotin is a biotin-labeled SNAP tag probe. SNAP-Biotin can be used for protein biotinylation .

Biotinylated Rapid Labeling Kit
The Biotinylation Rapid Labelling Kit enables biotinylation of proteins. Based on NHS ester chemistry, the NHS ester-activated fluorescent dye reacts with primary amines in the antibody/protein to be labelled within a pH 7-9 solution, forming stable amide bonds to achieve conjugation with the antibody/protein. Typically, a single IgG molecule can bind 2-8 molecules of biotin. The entire procedure can be completed within two hours.

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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