SNAP-Biotin
SNAP-Biotin is a cell-permeable SNAP-tag-targeted labeling reagent. SNAP-Biotin undergoes an irreversible covalent reaction with SNAP-tag to transfer biotin to the tag. SNAP-Biotin enables the detection of SNAP-tag fusion proteins via a streptavidin-HRP immunoblotting system and achieves biotinylation of SNAP-tag fusion proteins. SNAP-Biotin is applicable to triple-negative breast cancer-related research.
For research use only. We do not sell to patients.
- CAS No.: 471918-16-8
- Formula: C29H39N9O4S
- Molecular Weight:609.74
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
SNAP-Biotin enables the detection of SNAP-tagged proteins in SDS-PAGE/immunoblotting assays via streptavidin-HRP and enhanced chemiluminescence[1].
SNAP-Biotin (150 nM-1.5 mM) binds to the inactive SNAP-tag mutant SNAPC145A with measurable affinity[2].
SNAP-Biotin (1.5 μM; 30 min incubation, 11 h chase) labels parental H3.3-SNAP proteins in mouse embryonic stem cells[3].
SNAP-Biotin (1.5 μM; 30 min incubation, 14 h chase) labels parental H3.3-SNAP in mouse embryonic stem cells, where Mcm2-Pola1 and Pole3-Pole4 promote the transfer of parental H3.3-SNAP to the lagging strand and leading strand of DNA replication, respectively[3].
SNAP-Biotin (30 μM; 1 h) specifically labels the HCC1 fusion protein with a functional C-terminal SNAP tag in crude mitochondrial extracts from transgenic Arabidopsis hcc1-1 mutants[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 471918-16-8
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Appearance Solid
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Molecular Weight 609.74
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Formula C29H39N9O4S
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Color Off-white to light yellow
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SMILES
NC1=NC(OCC(C=C2)=CC=C2CNC(CCCCCNC(CCCC[C@H]3[C@]4([H])[C@](NC(N4)=O)([H])CS3)=O)=O)=C5C(N=CN5)=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Stoops EH, et al. SNAP-tag to monitor trafficking of membrane proteins in polarized epithelial cells. Methods in molecular biology (Clifton, N.J.). 2014;1174:171-82. [Content Brief]
[2]. Wilhelm J, et al. Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry. 2021 Aug 24;60(33):2560-2575. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SNAP-Biotin Related Classifications
HY-148958 Related Classifications
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