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reverse-phase HPLC

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By Targets:	Biochemical Assay Reagents (1) Endogenous Metabolite (1) FAAH (1) Fluorescent Dye (1)
By Research Areas:	Cancer (1) Metabolic Disease (2)

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Fluorescent Dyes

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Products

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-137899

Dephospho-CoA 1 Publications Verification	Endogenous Metabolite	Metabolic Disease Cancer
Dephospho-CoA is a key intermediate in the biosynthesis of coenzyme A (CoA), which is catalyzed to form CoA by GTP-dependent Dephospho-CoA kinase (DPCK). Dephospho-CoA completes the final synthetic step of CoA via phosphorylation and participates in energy metabolism and cellular signal transduction. Dephospho-CoA can be used in studies involving cancer (e.g., regulation of cell proliferation) and metabolic diseases (e.g., mitochondrial dysfunction) .

HY-118210

PHOP	FAAH	Metabolic Disease
PHOP is a fatty acid amide hydrolase (FAAH) inhibitor used to assess inhibitory activity in a fluorometric assay. PHOP can determine FAAH activity by measuring the amount of 4-pyridin-1-ylbutyric acid released by the enzyme in rat brain microsomes. PHOP demonstrates potential as a FAAH inhibitor and can directly measure FAAH activity by reversed-phase HPLC and fluorescence detection, providing a basis for the development of new inhibitors.

HY-120512

5-Phenyl-4E-pentenol	Fluorescent Dye	Others
5-Phenyl-4E-pentenol is a product of the peroxidase-catalyzed reduction of PPHP. 5-Phenyl-4E-pentenol can be rapidly isolated by solid phase extraction and quantified by isocratic reversed-phase high performance liquid chromatography (HPLC) with UV detection. 5-Phenyl-4E-pentenol is a fluorescent dye that can be used to determine the kinetic properties of heme-containing and non-heme peroxidases and to evaluate the ability of oxidative compounds to serve as substrates for peroxidase reduction. .

HY-166113A

Undecaprenyl-mpda monophosphate diammonium	Biochemical Assay Reagents	Others
Undecaprenyl-mpda monophosphate diammonium is an ester compounds that closely related to bacterial cell wall synthesis .

Cat. No.	Product Name	Type

HY-120512

5-Phenyl-4E-pentenol	Fluorescent Dyes
5-Phenyl-4E-pentenol is a product of the peroxidase-catalyzed reduction of PPHP. 5-Phenyl-4E-pentenol can be rapidly isolated by solid phase extraction and quantified by isocratic reversed-phase high performance liquid chromatography (HPLC) with UV detection. 5-Phenyl-4E-pentenol is a fluorescent dye that can be used to determine the kinetic properties of heme-containing and non-heme peroxidases and to evaluate the ability of oxidative compounds to serve as substrates for peroxidase reduction. .

5-Phenyl-4E-pentenol

Fluorescent Dyes

5-Phenyl-4E-pentenol is a product of the peroxidase-catalyzed reduction of PPHP. 5-Phenyl-4E-pentenol can be rapidly isolated by solid phase extraction and quantified by isocratic reversed-phase high performance liquid chromatography (HPLC) with UV detection. 5-Phenyl-4E-pentenol is a fluorescent dye that can be used to determine the kinetic properties of heme-containing and non-heme peroxidases and to evaluate the ability of oxidative compounds to serve as substrates for peroxidase reduction. .

Dephospho-CoA 1 Publications Verification	Structural Classification Natural Products Human Gut Microbiota Metabolites Classification of Application Fields Other Diseases Endogenous metabolite Disease Research Fields Source Classification	Endogenous Metabolite
Dephospho-CoA is a key intermediate in the biosynthesis of coenzyme A (CoA), which is catalyzed to form CoA by GTP-dependent Dephospho-CoA kinase (DPCK). Dephospho-CoA completes the final synthetic step of CoA via phosphorylation and participates in energy metabolism and cellular signal transduction. Dephospho-CoA can be used in studies involving cancer (e.g., regulation of cell proliferation) and metabolic diseases (e.g., mitochondrial dysfunction) .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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reverse-phase HPLC

Inhibitors & Agonists

Fluorescent Dyes

NaturalProducts

Natural
Products