2. NF-κB MAPK/ERK Pathway Metabolic Enzyme/Protease
  3. NF-κB p38 MAPK MMP
  4. SM-7368

SM-7368 is a potent NF-kB inhibitor that targets downstream of MAPK p38 activation. SM-7368 inhibits TNF-α-induced MMP-9 upregulation. SM-7368 can be used for the research of chemotherapies targeting TNF-α-mediated tumor invasion and metastasis .

For research use only. We do not sell to patients.

SM-7368 Chemical Structure

SM-7368 Chemical Structure

CAS No. : 380623-76-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 95 In-stock
Solution
10 mM * 1 mL in DMSO USD 95 In-stock
Solid
1 mg USD 35 In-stock
5 mg USD 86 In-stock
10 mg USD 140 In-stock
25 mg USD 280 In-stock
50 mg USD 420 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

SM-7368 Related Antibodies

Top Publications Citing Use of Products

View All NF-κB Isoform Specific Products:

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

View All MMP Isoform Specific Products:

View All Isoforms
MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SM-7368 is a potent NF-kB inhibitor that targets downstream of MAPK p38 activation[1]. SM-7368 inhibits TNF-α-induced MMP-9 upregulation. SM-7368 can be used for the research of chemotherapies targeting TNF-α-mediated tumor invasion and metastasis [2].

IC50 & Target[1][2]

NF-κB

 

MMP-9

 

p38 MAP kinase

 

In Vitro

SM-7368 (5 μM) targets downstream of MAPK p38 activation in the human colon derived crypt like HT-29 and Caco-2 epithelial cell lines[1].
SM-7368 inhibits TNF-α-induced MMP-9 upregulation in a concentration-dependent manner and shows maximal activity at 10 μM. SM-7368 inhibits TNF-α-induced MMP-9 mRNA transcript accumulation and protein expression. SM-7368 strongly inhibits TNF-α-induced NF-κB activity but not AP-1 activity. SM-7368 strongly inhibits the TNF-α-induced invasion of HT1080 human fibrosarcoma cell line[2].
SM-7368 (10-25 μM) greatly inhibits TNF-α (20 ng/mL)-induced MMP-9 upregulation. 10 μM of SM-7368 almost completely abrogates this upregulation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SM-7368 Related Antibodies

Western Blot Analysis[2]

Cell Line: HT1080 human fibrosarcoma cells
Concentration: 0, 1, 5, 10, 15, 20, and 25 μM
Incubation Time: 24 hours
Result: Greatly inhibited TNF-α (20 ng/mL)-induced MMP-9 upregulation in a concentration-dependent manner.
Molecular Weight

328.69

Formula

C10H5ClN4O5S
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

ClC1=C(C=CC(C(NC2=NC=C(S2)[N+]([O-])=O)=O)=C1)[N+]([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (253.52 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0424 mL 15.2119 mL 30.4238 mL
5 mM 0.6085 mL 3.0424 mL 6.0848 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.33 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.90%

COA (248 KB) HNMR (218 KB) LCMS (255 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0424 mL 15.2119 mL 30.4238 mL 76.0595 mL
5 mM 0.6085 mL 3.0424 mL 6.0848 mL 15.2119 mL
10 mM 0.3042 mL 1.5212 mL 3.0424 mL 7.6060 mL
15 mM 0.2028 mL 1.0141 mL 2.0283 mL 5.0706 mL
20 mM 0.1521 mL 0.7606 mL 1.5212 mL 3.8030 mL
25 mM 0.1217 mL 0.6085 mL 1.2170 mL 3.0424 mL
30 mM 0.1014 mL 0.5071 mL 1.0141 mL 2.5353 mL
40 mM 0.0761 mL 0.3803 mL 0.7606 mL 1.9015 mL
50 mM 0.0608 mL 0.3042 mL 0.6085 mL 1.5212 mL
60 mM 0.0507 mL 0.2535 mL 0.5071 mL 1.2677 mL
80 mM 0.0380 mL 0.1901 mL 0.3803 mL 0.9507 mL
100 mM 0.0304 mL 0.1521 mL 0.3042 mL 0.7606 mL
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SM-7368 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SM-7368380623-76-7SM7368SM 7368NF-κBp38 MAPKMMPNuclear factor-κBNuclear factor-kappaBMatrix metalloproteinasesMMP-9Matrixmetalloproteinase-9,Interleukin-8,p38MAPKTNF-αInvasionInhibitorinhibitorinhibit

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