1. JAK/STAT Signaling Stem Cell/Wnt Anti-infection
  2. STAT Parasite Antibiotic
  3. Niclosamide

Niclosamide  (Synonyms: BAY2353)

Cat. No.: HY-B0497 Purity: 99.90%
COA Handling Instructions

Niclosamide (BAY2353) is an orally active antihelminthic agent used in parasitic infection research. Niclosamide is a STAT3 inhibitor with an IC50 of 0.25 μM in HeLa cells. Niclosamide has biological activities against cancer, inhibits DNA replication in Vero E6 cells.

For research use only. We do not sell to patients.

Niclosamide Chemical Structure

Niclosamide Chemical Structure

CAS No. : 50-65-7

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10 mM * 1 mL in DMSO
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Customer Review

Based on 22 publication(s) in Google Scholar

Other Forms of Niclosamide:

Top Publications Citing Use of Products

    Niclosamide purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May.

    Niclosamide (NIC; 1 μM) reduces number of cells that invaded across the trans-well chamber in Hec1B and Ishikawa endometrial cancer cell lines, suggesting NIC-mediated inhibition of invasive property of endometrial cancer cells.

    Niclosamide purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May.

    Niclosamide (NIC) significantly suppresses the colony formation ability, and reduces the numbers, as well as diameters, of colonies formed in AN3CA, Hec1B, and Ishikawa cells.

    Niclosamide purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May.

    Niclosamide (NIC; 5 μM; 24 h) significantly reduces cyclin D1 and vimentin protein expression in human endometrial cancer cells.

    Niclosamide purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May.

    Niclosamide (NIC; 0-8 μM; 24, 48, 72 h) significantly inhibits the metabolic viability of AN3CA, Hec1B, and Ishikawa cells both in a dose-dependent and a time-dependent manner, with the half maximal inhibitory concentration of < 1 μM at 72 h.
    • Biological Activity

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    • Customer Review

    Description

    Niclosamide (BAY2353) is an orally active antihelminthic agent used in parasitic infection research[1]. Niclosamide is a STAT3 inhibitor with an IC50 of 0.25 μM in HeLa cells[4]. Niclosamide has biological activities against cancer, inhibits DNA replication in Vero E6 cells[2][3][5].

    IC50 & Target

    STAT3

    0.25 μM (IC50, in HeLa cells)

    In Vitro

    Niclosamide (0.6 nM-46 μM) treatment inhibits adrenocortical carcinoma cellular proliferation in BD140A, SW-13, and NCI-H295R cells[3].
    Niclosamide (0.05-5 μM, 24 h) treatment inhibits STAT3-mediated luciferase reporter activity in HeLa cells [4].
    Niclosamide (10 μM) treatment inhibits virus replication in Vero E6 cells[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[3]

    Cell Line: BD140A, SW-13 and NCI-H295R cells
    Concentration: 0.6 nM-46 µM
    Incubation Time:
    Result: Inhibited cellular proliferation in adrenocortical carcinoma cell lines with the IC50 of 0.12 µM, 0.15 µM, and 0.53 µM in BD140A, SW-13, and NCI-H295R, respectively.

    Cell Viability Assay[4]

    Cell Line: Hela cells
    Concentration: 0.05-5 μM
    Incubation Time: 24 hours
    Result: Inhibited STAT3-mediated luciferase reporter activity with an IC50 of 0.25 μM.

    Cell Viability Assay[4]

    Cell Line: Vero E6 cells[5]
    Concentration: 10 μM
    Incubation Time: 2 days
    Result: Inhibited the synthesis of viral antigens of SARS-CoV in Vero E6 cells.
    In Vivo

    Niclosamide (oral gavage; 100 mg/kg, 200 mg/kg; once a week; 8 weeks) treatment inhibits adrenocortical carcinoma tumor growth in vivo[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Nu+/Nu+ mice injected with NCI-H295R cells[3]
    Dosage: 100 mg/kg, 200 mg/kg
    Administration: Oral gavage; 100 mg/kg, 200 mg/kg; once a week; 8 weeks
    Result: Showed a 60%-80% inhibition in tumor growth, as compared to the control group.
    Clinical Trial
    Molecular Weight

    327.12

    Formula

    C13H8Cl2N2O4

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to green yellow

    SMILES

    O=C(NC1=CC=C([N+]([O-])=O)C=C1Cl)C2=CC(Cl)=CC=C2O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMF : 5 mg/mL (15.28 mM; Need ultrasonic)

    DMSO : 4.55 mg/mL (13.91 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.0570 mL 15.2849 mL 30.5698 mL
    5 mM 0.6114 mL 3.0570 mL 6.1140 mL
    10 mM 0.3057 mL 1.5285 mL 3.0570 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  20% HP-β-CD    5% Cremophor EL

      Solubility: 5 mg/mL (15.28 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMF    40% PEG300    5% Tween-80    45% Saline

      Solubility: 0.5 mg/mL (1.53 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.90%

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Niclosamide
    Cat. No.:
    HY-B0497
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