Sulfo Cy5.5-N3
Sulfo Cy5.5-N3 is a Sulfo Cy5.5-azide, which is a fluorescent dye. Sulfo Cy5.5-N3 can be used in cell imagine by Click reaction. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- Formula: C46H53N6NaO10S3
- Molecular Weight:969.13
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Chemical Information
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Molecular Weight 969.13
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Formula C46H53N6NaO10S3
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SMILES
CC(C1=C(N/2CCCCS(=O)(O)=O)C=CC3=C1C=CC(S(=O)(O[Na])=O)=C3)(C)C2=C/C=C/C=C/C4=[N+](CCCCCC(NCCCN=[N+]=[N-])=O)C(C=CC5=C6C=CC(S(=O)([O-])=O)=C5)=C6C4(C)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Directly Induced Neuron Culture
Directly induced neuron culture converts somatic cells, most commonly fibroblasts, into induced neurons without passing through a pluripotent or neural progenitor stage; classic evidence shows that mouse fibroblasts can be converted by Ascl1, Brn2/Pou3f2, and Myt1l, human fibroblasts can be converted by defined neuronal transcription factors, and human fibroblasts can also be converted by miR-9/9-124 with neurogenic or subtype-specifying transcription factors. The readout is acquisition of neuronal identity and function, assessed by neuronal morphology, neuronal markers such as Tuj1/βIII-tubulin, MAP2, synapsin, and subtype markers when relevant, together with functional assays such as action-potential firing, synaptic activity, and electrophysiology.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)