Sulfo-Cy5-Methyltetrazine
Sulfo-Cy5-Methyltetrazine is a click chemistry reagent containing a methyltetrazine group. Sulfo-Cy5-Methyltetrazine acts as a fluorophore linker for trans-cyclooctene-based labeling. Sulfo-Cy5-Methyltetrazine shows good stability at physiological pH and is also highly reactive towards cyclooctene.
For research use only. We do not sell to patients.
- CAS No.: 1801924-46-8
- Formula: C44H51N7O10S3
- Molecular Weight:934.11
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
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HY-D1482
Sulfo-Cy5-tetrazineCy5 Derivative -
HY-D1068
Cy5-DBCOFluorescent Dye -
HY-D1069
DBCO-Cy3Orange Fluorescent Dye -
HY-D1880
Sulfo-Cy5 hydrazideFluorescent Dye -
HY-151710
Sulfo-Cy3 AzideFluorogenic Azide Dye -
HY-145746
Sulfo-Cy5 azideFluorescent Dye -
HY-151713
Sulfo-Cy3-MethyltetrazineClick Chemistry Reagent -
HY-D1390
Sulfo-Cy5-Mal potassiumFluorescent Dye
Biological Activity
Description
Emission (Em)
668
Excitation (Ex)
647
Chemical Information
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CAS No. 1801924-46-8
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Molecular Weight 934.11
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Formula C44H51N7O10S3
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SMILES
O=C(CCCCC[N+]1=C(/C=C/C=C/C=C2N(CCCS(=O)(O)=O)C3=CC=C(S(=O)(O)=O)C=C3C\2(C)C)C(C)(C)C4=C1C=CC(S(=O)([O-])=O)=C4)NCC5=CC=C(C6=NN=C(C)N=N6)C=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Sulfo-Cy5-Methyltetrazine Related Classifications
HY-151712 Related Classifications
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