Sulfo DBCO-UBQ-1
Sulfo DBCO-UBQ-1 is a click chemistry reagent uniting UBQ-1, which is a dark quencher with a polyaromatic-azo backbone that exhibits no native emission, with a sulfo group for improved hydrophilicity and DBCO for undergoing copper-free click chemistry.
For research use only. We do not sell to patients.
- Formula: C47H47N9O9S
- Molecular Weight:914.00
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
-
Help & FAQs
Biological Activity
Description
Chemical Information
-
Molecular Weight 914.00
-
Formula C47H47N9O9S
-
SMILES
CC(C=C1[N+]([O-])=O)=CC=C1/N=N/C(C=C2OC)=C(C)C=C2/N=N/C(C=C3)=CC=C3N(C)CCCC(NCC(S(=O)(O)=O)C(NCCC(N4CC5=C(C=CC=C5)C#CC6=C4C=CC=C6)=O)=O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Sulfo DBCO-UBQ-1 Related Classifications
HY-D3322 Related Classifications
Powered by Bioz