TAMRA DBCO, 5-isomer 

TAMRA DBCO, 5-isomer is a derivative of TAMRA fluorescent isomer with a DBCO functional group. TAMRA DBCO, 5-isomer undergoes strain-promoted azide-alkyne cycloaddition (SPAAC) under copper-free catalytic conditions. TAMRA DBCO, 5-isomer serves as a FRET acceptor for FAM fluorophores. TAMRA DBCO, 5-isomer can label proteins, peptides, nucleic acids and all azide-modified biomolecules, and is suitable for fluorescence imaging and flow cytometry to quantify intracellular SPAAC reactions^[1].

TAMRA DBCO, 5-isomer is a derivative of tetramethylrhodamine (TMR, TAMRA) that contains a cyclooctyne moiety (dibenzocyclooctyne, DBCO). DBCO reacts rapidly and efficiently with azides simply by mixing the components, without the need for a copper catalyst (i.e., strain-promoted azide-alkyne cycloaddition (SPAAC))^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

730.85

C₄₆H₄₂N₄O₅

1911598-65-6

O=C(C1=C(C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)C=CC(C(NCCCCCC(N5C6=C(C#CC7=C(C5)C=CC=C7)C=CC=C6)=O)=O)=C1)[O-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kovacs L, et al. Development of an In Vitro Assay for Evaluation of Intracellular SPAAC Reactions Using Hoechst-DBCO. Chem Biomed Imaging. 2025;4(5):912-923.  [Content Brief]